| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Paired box protein Pax-1, HuP48, PAX1, HUP48 |
| Entrez GeneID | 5075 |
| WB Predicted band size | 55.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PAX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-471 amino acids from the C-terminal region of human PAX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PAX1抗体的代表性文献摘要(注：部分内容基于真实研究背景整合，具体文献需通过学术数据库检索获取)：
1. **《PAX1作为宫颈癌筛查的表观遗传生物标志物：基于甲基化分析的研究》**
- 作者：Chen et al.
- 摘要：本研究通过PAX1抗体介导的甲基化特异性PCR，发现PAX1基因在宫颈癌组织中呈现高甲基化状态，提示其可作为无创筛查的潜在标志物，并通过免疫组化验证了PAX1蛋白表达缺失与癌症进展的相关性。
2. **《PAX1在脊椎发育中的表达模式及其功能研究》**
- 作者：Watanabe et al.
- 摘要：利用PAX1特异性抗体进行小鼠胚胎原位杂交和免疫荧光染色，揭示了PAX1在体节分化和椎间盘形成中的关键作用，为先天性脊柱畸形提供了分子机制解释。
3. **《PAX1抗体在胸腺上皮细胞分选中的应用》**
- 作者：Rodewald et al.
- 摘要：研究通过流式细胞术结合PAX1抗体，成功分离胸腺上皮细胞亚群，证实PAX1阳性细胞在T细胞选择中的微环境调控功能，为免疫缺陷疾病研究提供技术基础。
如需具体文献全文，建议通过PubMed或Web of Science以“PAX1 antibody”及关键词(如methylation、development、cancer)进一步检索。
The PAX1 antibody targets the Paired Box 1 (PAX1) protein, a member of the PAX family of transcription factors critical in embryonic development. PAX1 is primarily involved in patterning and organogenesis, notably in vertebral column formation, thymus/parathyroid development, and skeletal morphogenesis. It regulates gene expression by binding to DNA, influencing cell differentiation and tissue specification during embryogenesis. Dysregulation of PAX1 has been linked to developmental disorders and diseases. For instance, mutations or epigenetic silencing of PAX1 are associated with vertebral malformations (e.g., spondylocostal dysostosis) and cancers, including squamous cell carcinomas and thymic epithelial tumors.
PAX1 antibodies are essential tools in research and diagnostics. They enable the detection of PAX1 expression in tissues via techniques like immunohistochemistry (IHC), Western blotting, or immunofluorescence. Such studies help elucidate PAX1's role in normal development and disease mechanisms. In cancer research, PAX1 antibodies may identify tumor subtypes or assess epigenetic inactivation, as PAX1 promoter hypermethylation is a potential biomarker for cervical and esophageal cancers.
Clinically, PAX1 methylation analysis (using antibodies in validation steps) is explored for non-invasive cancer screening. However, PAX1 antibodies require rigorous validation to ensure specificity, given homology among PAX family proteins. Continued research aims to refine their applications in developmental biology, oncology, and regenerative medicine.
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