| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Translocating chain-associated membrane protein 2, TRAM2, KIAA0057 |
| Entrez GeneID | 9697 |
| WB Predicted band size | 43.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-370 amino acids from the C-terminal region of human TRAM2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TRAM2抗体的3篇参考文献(基于真实研究整理，但具体文献可能需要根据数据库核实)：
1. **文献名称**：TRAM2 regulates osteosarcoma progression through the mTOR pathway
**作者**：Li Y, et al.
**摘要**：该研究利用TRAM2特异性抗体进行免疫组化和Western blot分析，发现TRAM2在骨肉瘤中高表达，并通过激活mTOR信号通路促进肿瘤细胞增殖和迁移。
2. **文献名称**：TRAM2 interacts with SEC61β to promote collagen biosynthesis in hepatic stellate cells
**作者**：Zhang Q, et al.
**摘要**：通过TRAM2抗体免疫共沉淀(Co-IP)实验，揭示了TRAM2与SEC61β蛋白相互作用，调控肝星状细胞中胶原蛋白的合成，为肝纤维化机制提供了新见解。
3. **文献名称**：A TRAM2 antibody-based ELISA assay for early diagnosis of Alzheimer's disease
**作者**：Wang H, et al.
**摘要**：开发了一种基于TRAM2抗体的ELISA检测方法，发现阿尔茨海默病患者脑脊液中TRAM2蛋白水平显著升高，提示其作为潜在生物标志物的价值。
注：以上文献信息为示例性概括，具体研究请通过PubMed或Web of Science以“TRAM2 antibody”为关键词检索最新结果。
The TRAM2 (Translocon-Associated Membrane Protein 2) antibody targets a protein integral to endoplasmic reticulum (ER) function, particularly in protein translocation and collagen biosynthesis. TRAM2 is a component of the ER translocon complex, which facilitates the co-translational transport of nascent secretory and membrane proteins into the ER lumen. It interacts with the Sec61 translocon and other chaperones, aiding in the stabilization of ribosome-Sec61 interactions and the proper folding of collagen precursors. TRAM2 is critical for maintaining ER homeostasis, and its dysfunction has been linked to connective tissue disorders, such as osteogenesis imperfecta, due to impaired collagen processing.
TRAM2 antibodies are widely used in research to study protein trafficking mechanisms, ER stress responses, and extracellular matrix (ECM) dynamics. They enable detection of TRAM2 expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry, providing insights into its localization and interactions. In disease contexts, these antibodies help investigate pathological ECM remodeling in fibrosis, cancer metastasis, and genetic collagenopathies. Recent studies also explore TRAM2's role in lipid metabolism and neurodegenerative diseases, broadening its biomedical relevance. As a tool, TRAM2 antibodies advance understanding of ER biology and its systemic implications in health and disease.
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