| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | U6 snRNA-associated Sm-like protein LSm4, Glycine-rich protein, GRP, LSM4 |
| Entrez GeneID | 25804 |
| WB Predicted band size | 15.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LSM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-74 amino acids from the Central region of human LSM4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LSM4抗体的3篇文献示例(内容基于公开文献概括，非真实引用)：
1. **文献名称**：*LSM4 interacts with hepatitis C virus NS5B and regulates viral replication*
**作者**：Li X, et al.
**摘要**：本研究利用LSM4特异性抗体进行免疫共沉淀实验，发现LSM4与丙型肝炎病毒NS5B蛋白直接结合，调控病毒RNA复制过程。敲低LSM4显著抑制病毒复制，提示其作为潜在抗病毒靶点。
2. **文献名称**：*The role of LSM4 in stress granule assembly and mRNA stability*
**作者**：Wang Y, et al.
**摘要**：通过LSM4抗体的免疫荧光分析，证实LSM4在细胞应激条件下(如氧化应激)被招募至应激颗粒(stress granules)，并参与维持mRNA稳定性。研究揭示了LSM4在细胞应激反应中的关键作用。
3. **文献名称**：*Proteomic analysis of LSM4-containing complexes in human cells*
**作者**：Zhang R, et al.
**摘要**：利用LSM4抗体进行免疫沉淀结合质谱分析，鉴定了LSM4在细胞中与多种剪接体(spliceosome)蛋白的相互作用网络，为LSM家族蛋白在RNA加工中的分子机制提供了新证据。
4. **文献名称**：*LSM4 expression correlates with poor prognosis in breast cancer*
**作者**：Chen H, et al.
**摘要**：通过LSM4抗体的免疫组化分析，发现LSM4在乳腺癌组织中高表达，并与患者生存率负相关。功能实验表明，LSM4通过调控致癌基因mRNA的稳定性促进肿瘤进展。
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**注**：以上为模拟内容，实际文献需通过PubMed或Google Scholar检索关键词“LSM4 antibody”获取。如需具体文献，可提供数据库访问权限进一步筛选。
The LSM4 antibody is a tool used to study the LSM4 protein, a member of the Like Sm (LSM) family involved in RNA metabolism. LSM proteins are highly conserved and form ring-shaped complexes that bind RNA, participating in processes like mRNA splicing, degradation, and translation. Specifically, LSM4 is a core component of the cytoplasmic LSM1-7 complex, which facilitates mRNA decapping and 5’-to-3’ exonucleolytic decay by recruiting enzymes like DCP1/DCP2. It also associates with nuclear LSM2-8 complexes, contributing to U6 snRNA stability in spliceosomes.
LSM4 antibodies are critical for detecting the protein’s expression, localization, and interactions in cellular models. Researchers employ these antibodies in techniques such as Western blotting, immunofluorescence, and co-immunoprecipitation to explore LSM4’s role in RNA regulation and stress responses. Dysregulation of LSM4 has been linked to diseases like cancer and neurodegenerative disorders, where aberrant RNA processing is implicated. For instance, reduced LSM4 levels may disrupt mRNA turnover, potentially promoting oncogenesis or neuronal dysfunction.
Studies using LSM4 antibodies have also shed light on its interplay with other RNA-binding proteins and its involvement in cellular pathways like apoptosis and cell cycle control. These insights highlight LSM4’s importance in maintaining RNA homeostasis and its potential as a therapeutic target. Overall, LSM4 antibodies serve as vital reagents for unraveling the molecular mechanisms of RNA metabolism and related pathologies.
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