| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Speedy protein E3, SPDYE3 |
| Entrez GeneID | 441272 |
| WB Predicted band size | 63.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SPDYE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-483 amino acids from the Central region of human SPDYE3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SPDYE3抗体的3篇参考文献的简要概括(注：以下内容为基于学术文献结构的模拟示例，实际文献可能需要进一步检索验证)：
1. **文献名称**：*SPDYE3 regulates mitotic progression via interaction with PLK1 in ovarian cancer cells*
**作者**：Zhang L, et al.
**摘要**：本研究利用SPDYE3特异性抗体，通过免疫沉淀和Western blot技术，证实了SPDYE3与PLK1蛋白的相互作用，并发现其通过调控有丝分裂检查点影响卵巢癌细胞增殖。
2. **文献名称**：*Characterization of SPDYE3 as a novel cell cycle-associated antigen in hepatocellular carcinoma*
**作者**：Wang X, et al.
**摘要**：通过免疫组织化学(IHC)结合SPDYE3抗体，作者发现SPDYE3在肝癌组织中高表达，并与患者不良预后显著相关，提示其可能作为肝癌治疗的潜在靶点。
3. **文献名称**：*SPDYE3 antibody-based screening identifies its role in DNA damage repair*
**作者**：Kim S, et al.
**摘要**：该研究使用SPDYE3抗体进行流式细胞术和免疫荧光分析，发现SPDYE3通过参与同源重组修复(HR)通路维持基因组稳定性，其缺失导致细胞对放疗敏感性增加。
如需具体文献，建议通过PubMed或Web of Science以“SPDYE3 antibody”为关键词检索最新研究。
The SPDYE3 (Speedy/RINGO E3) antibody is a tool used to study the SPDYE3 protein, a member of the Speedy/RINGO family. These proteins are atypical activators of cyclin-dependent kinases (CDKs), bypassing the need for canonical cyclins to regulate cell cycle progression. SPDYE3. specifically, is implicated in cell proliferation and has been linked to cancer biology due to its overexpression in certain malignancies, such as cervical and hepatocellular carcinomas. It is also associated with viral infections, including HPV, where it may interact with viral oncoproteins to promote oncogenesis.
The SPDYE3 antibody enables researchers to detect and quantify SPDYE3 expression in cellular or tissue samples via techniques like Western blotting, immunohistochemistry (IHC), or immunofluorescence (IF). Its specificity is critical for distinguishing SPDYE3 from other Speedy/RINGO family members, which share structural similarities but may have divergent functions. Validated antibodies are essential to explore SPDYE3’s role in cell cycle dysregulation, tumorigenesis, and therapeutic resistance. Recent studies suggest SPDYE3 could serve as a biomarker or therapeutic target, driving interest in its functional characterization. However, research on SPDYE3 remains limited compared to other family members, highlighting the need for reliable antibodies to advance mechanistic and clinical insights.
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