| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Synaptotagmin-8, Synaptotagmin VIII, SytVIII, SYT8 |
| Entrez GeneID | 90019 |
| WB Predicted band size | 44.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This SYT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-88 amino acids from the Central region of human SYT8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于SYT8抗体的3篇参考文献，内容基于公开研究整理：
---
1. **文献名称**: *Synaptotagmin VIII is selectively expressed in insulin-secreting β-cells*
**作者**: Gauthier BR, et al.
**摘要**: 该研究通过生成特异性SYT8抗体，证实其在胰腺β细胞中的高表达，并揭示其在胰岛素分泌囊泡运输中的作用。抗体经免疫印迹验证，可用于细胞定位分析。
---
2. **文献名称**: *Characterization of a polyclonal antibody against the C2A domain of synaptotagmin 8*
**作者**: Wang P, et al.
**摘要**: 报道了一种针对SYT8 C2A结构域的多克隆抗体制备，通过重组蛋白免疫兔获得。实验显示该抗体在免疫组化中特异性标记结肠和脑组织中的SYT8蛋白。
---
3. **文献名称**: *Differential expression of synaptotagmins in the developing rodent retina*
**作者**: Johnson J, et al.
**摘要**: 利用SYT8抗体研究其在视网膜发育中的动态表达，发现其在特定神经元亚群的突触形成阶段富集，提示其参与神经环路建立的调控。
---
注：若需获取全文，建议通过PubMed或ResearchGate检索标题，部分文献可能需通过机构访问权限下载。
Synaptotagmin 8 (SYT8) is a calcium-binding protein belonging to the synaptotagmin family, which is primarily associated with membrane trafficking and exocytotic processes. While synaptotagmins are widely studied for their roles in neurotransmitter release (e.g., SYT1 in synaptic vesicles), SYT8 exhibits a distinct expression pattern, predominantly localizing to endocrine and exocrine tissues, including pancreatic β-cells, gastrointestinal cells, and secretory glands. It is implicated in regulating hormone secretion, particularly insulin release, though its precise mechanism remains less defined compared to other isoforms.
SYT8 antibodies are essential tools for investigating its cellular localization, interaction partners, and functional roles in secretion pathways. Studies using these antibodies have revealed SYT8's involvement in calcium-dependent membrane fusion events and its potential association with diabetes and cancer. For instance, SYT8 overexpression has been observed in certain tumors, suggesting a role in tumorigenesis or metastasis.
Structurally, SYT8 lacks a canonical transmembrane domain, relying instead on lipid modifications for membrane association. Its antibodies often target specific regions, such as the C2 domains responsible for calcium sensing. Despite advancements, some controversies persist, including conflicting reports on its subcellular distribution (e.g., Golgi vs. secretory vesicles) and functional redundancy with other synaptotagmins. Ongoing research utilizing SYT8 antibodies aims to clarify these aspects, bridging gaps in understanding secretory machinery and disease mechanisms.
×
