Rabbit Polyclonal MAF1 Antibody

中文名： MAF1抗体
别名： Repressor of RNA polymerase III transcription MAF1 homolog, MAF1

货号: IPDX30740

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/1000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/100-1/500	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	1/10-1/50	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	EI; LEI; PI2; MNEI; PI-2; HEL57; M/NEI; ELANH2
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Fusion protein of human SERPINB1
Formulation	Purified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P01789(SERPINB1 Antibody) at dilution 1/30. (Original magnification: ×200)

参考文献

以下是3-4条关于MAF1抗体的参考文献示例(注：文献信息为模拟，仅供参考)：

1. **"MAF1 is a negative regulator of RNA polymerase III transcription"**

- **作者**: Cabarcas S, Schramm L (2011)

- **摘要**: 研究利用特异性MAF1抗体，通过免疫印迹和免疫沉淀技术，证明MAF1通过与RNA聚合酶III结合抑制转录活性，在细胞生长调控中起关键作用。

2. **"A conserved role for MAF1 in regulating mitochondrial metabolism"**

- **作者**: Johnson SS, et al. (2013)

- **摘要**: 通过Western blot和免疫荧光(使用MAF1抗体)，揭示MAF1通过mTOR信号通路调控线粒体功能，并参与能量应激条件下的代谢适应。

3. **"Development and validation of a high-affinity monoclonal antibody against human MAF1"**

- **作者**: Lee H, Kim J (2017)

- **摘要**: 描述了一种新型抗人MAF1单克隆抗体的开发，经ELISA和免疫组化验证其特异性，适用于检测多种组织中的MAF1蛋白表达。

4. **"MAF1 links cellular nutrient sensing to epigenetic regulation"**

- **作者**: Bonhoure N, et al. (2015)

- **摘要**: 研究利用MAF1抗体进行染色质免疫共沉淀(ChIP)，发现MAF1通过表观遗传机制调控核糖体RNA合成，响应营养可用性变化。

(注：实际文献需通过PubMed或Google Scholar以关键词“MAF1 antibody”或“MAF1 immunodetection”检索确认。)

背景信息

The MAF1 antibody is a crucial tool for studying the MAF1 protein, a conserved transcriptional repressor that regulates RNA polymerase III (Pol III)-mediated transcription. MAF1 (MACRO Domain Containing 1A) functions as a key integrator of cellular stress and nutrient signaling pathways, modulating Pol III activity in response to metabolic changes, DNA damage, or other stressors. By inhibiting Pol III, MAF1 controls the synthesis of tRNA, 5S rRNA, and other small non-coding RNAs, thereby influencing global protein synthesis, cell growth, and energy homeostasis. Dysregulation of MAF1 has been linked to cancer, metabolic disorders, and aging-related processes. The MAF1 antibody enables researchers to detect and quantify MAF1 expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation. It is widely used to investigate MAF1's role in cellular stress responses, mTOR signaling, and its interplay with oncogenes or tumor suppressors. Commercial MAF1 antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation often includes knockout cell line controls to ensure specificity. Researchers utilize these antibodies to explore MAF1's post-translational modifications, subcellular localization, and interactions with other regulatory proteins, advancing our understanding of its physiological and pathological mechanisms.