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Rabbit Polyclonal RFT1 Antibody

  • 中文名： RFT1抗体
  • 别    名： Protein RFT1 homolog, RFT1
货号: IPDX30728
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesProtein RFT1 homolog, RFT1
Entrez GeneID91869
WB Predicted band size60.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis RFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-541 amino acids from the C-terminal region of human RFT1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于RFT1抗体的3-4条参考文献示例(内容为模拟生成，仅供参考)：

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1. **文献名称**：RFT1 mutations disrupt riboflavin transport and cause Brown-Vialetto-Van Laere syndrome.

**作者**：Green P, et al.

**摘要**：本研究利用RFT1特异性抗体，通过免疫荧光和Western blot技术，揭示了RFT1基因突变导致核黄素转运功能障碍的分子机制，为相关神经退行性疾病的诊断提供依据。

2. **文献名称**：Characterization of a monoclonal antibody against human RFT1 for detecting riboflavin transporter expression.

**作者**：Yonezawa A, Inui K.

**摘要**：报道了一种高特异性RFT1单克隆抗体的开发，并验证其在免疫组化和流式细胞术中的应用，证实RFT1在肠上皮细胞和癌细胞中的差异表达。

3. **文献名称**：RFT1 deficiency alters cellular redox homeostasis via impaired riboflavin uptake.

**作者**：Udhayabanu T, et al.

**摘要**：通过RFT1抗体检测发现，RFT1缺陷细胞中核黄素摄取减少，导致线粒体氧化代谢异常和氧化应激水平升高，提示其与代谢疾病的潜在关联。

4. **文献名称**：RFT1 antibody-based biomarker panel for early Alzheimer's disease detection.

**作者**：Johnson JO, et al.

**摘要**：研究利用RFT1抗体结合其他标志物，构建阿尔茨海默病早期诊断模型，发现脑脊液中RFT1蛋白水平与疾病进展显著相关。

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注：以上文献信息为模拟示例，实际引用请通过学术数据库(如PubMed、Web of Science)检索并核实原文。

背景信息

The RFT1 antibody is a crucial tool for studying the RFT1 protein, a conserved endoplasmic reticulum (ER)-resident transmembrane protein involved in lipid-linked oligosaccharide (LLO) biosynthesis. RFT1 facilitates the translocation (flipping) of Man₅GlcNAc₂-PP-dolichol, a key intermediate in N-linked glycosylation, from the cytoplasmic to the luminal side of the ER membrane. This process is essential for proper protein glycosylation, which affects protein folding, trafficking, and cellular communication. Mutations in the *RFT1* gene are linked to congenital disorders of glycosylation (CDG), specifically CDG type 1N (CDG1N), characterized by developmental delays, neurological deficits, and multi-organ dysfunction. The RFT1 antibody enables detection and localization of RFT1 in various experimental models (e.g., Western blot, immunofluorescence), aiding research on glycosylation mechanisms and disease pathogenesis. Its applications extend to screening for RFT1 expression anomalies in clinical samples and validating cellular/murine models of CDG. Studies using this antibody have also explored RFT1's interaction with other glycosylation machinery components, providing insights into ER flippase complexes. As glycosylation defects are implicated in cancers, immune disorders, and neurodegenerative diseases, the RFT1 antibody serves as a vital reagent for both basic research and translational studies targeting glycosylation-related pathologies.

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