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Rabbit Polyclonal TMEM214(N-term) Antibody

  • 中文名: TMEM214 (N-term)抗体
  • 别    名: Transmembrane protein 214, TMEM214
货号: IPDX30727
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

参考文献

以下是关于TMEM214 (N-term)抗体的3篇参考文献的简要整理:

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1. **文献名称**:*TMEM214 mediates ER-associated degradation involved in hepatic steatosis*

**作者**:Li, X. et al.

**摘要**:该研究通过Western blot和免疫荧光技术,利用TMEM214 (N-term)抗体验证了TMEM214蛋白在内质网相关降解(ERAD)中的作用。研究发现,TMEM214与内质网应激相关蛋白互作,其表达水平在非酒精性脂肪肝模型中显著上调。

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2. **文献名称**:*Antibody-based profiling of transmembrane protein TMEM214 in cancer cell lines*

**作者**:Smith, J.R. et al.

**摘要**:文章开发并验证了一种靶向TMEM214 N端表位的单克隆抗体,用于检测多种癌细胞系中TMEM214的膜定位。通过流式细胞术和免疫组化证实了该抗体的特异性,并发现TMEM214在胶质母细胞瘤中高表达。

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3. **文献名称**:*Functional characterization of TMEM214 in mitochondrial apoptosis*

**作者**:Wang, Y. et al.

**摘要**:研究使用TMEM214 (N-term)抗体探究该蛋白在线粒体凋亡通路中的作用。通过敲除实验和免疫共沉淀发现,TMEM214与Bcl-2家族蛋白互作,调控细胞凋亡。抗体特异性通过siRNA敲低后的Western blot验证。

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**备注**:上述文献为示例,实际文献需通过PubMed、Google Scholar或抗体公司官网(如CST、Abcam)查询。部分公司(如Sigma-Aldrich)的TMEM214抗体产品页面可能提供相关引用文献。

背景信息

The TMEM214 (N-term) antibody targets the N-terminal region of Transmembrane Protein 214 (TMEM214), a poorly characterized protein encoded by the TMEM214 gene. TMEM214 is predicted to be a multi-pass transmembrane protein, potentially localized to the endoplasmic reticulum (ER) or Golgi apparatus. Its exact biological function remains unclear, though studies suggest involvement in ER stress responses, apoptosis regulation, or vesicular trafficking. The N-terminal-specific antibody enables researchers to detect full-length or truncated TMEM214 isoforms in applications like Western blotting, immunofluorescence, or immunoprecipitation. It has been utilized in studies exploring TMEM214's role in diseases, including cancer and neurodegenerative disorders, where ER stress pathways are dysregulated. Validation data typically include reactivity in human or model organism cell lines, with observed molecular weights around 30-40 kDa. However, limited commercial availability and functional characterization necessitate careful experimental optimization, including proper controls to confirm specificity. Recent interest in TMEM214 stems from its potential as a biomarker or therapeutic target in conditions linked to protein misfolding or cellular stress mechanisms, though further mechanistic studies are required to elucidate its precise physiological and pathological roles.

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