WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/10-1/50 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Transmembrane protein 214, TMEM214 |
Entrez GeneID | 54867 |
WB Predicted band size | 77.2kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This TMEM214 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-66 amino acids from the N-terminal region of human TMEM214. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于TMEM214 (N-term)抗体的3篇参考文献的简要整理:
---
1. **文献名称**:*TMEM214 mediates ER-associated degradation involved in hepatic steatosis*
**作者**:Li, X. et al.
**摘要**:该研究通过Western blot和免疫荧光技术,利用TMEM214 (N-term)抗体验证了TMEM214蛋白在内质网相关降解(ERAD)中的作用。研究发现,TMEM214与内质网应激相关蛋白互作,其表达水平在非酒精性脂肪肝模型中显著上调。
---
2. **文献名称**:*Antibody-based profiling of transmembrane protein TMEM214 in cancer cell lines*
**作者**:Smith, J.R. et al.
**摘要**:文章开发并验证了一种靶向TMEM214 N端表位的单克隆抗体,用于检测多种癌细胞系中TMEM214的膜定位。通过流式细胞术和免疫组化证实了该抗体的特异性,并发现TMEM214在胶质母细胞瘤中高表达。
---
3. **文献名称**:*Functional characterization of TMEM214 in mitochondrial apoptosis*
**作者**:Wang, Y. et al.
**摘要**:研究使用TMEM214 (N-term)抗体探究该蛋白在线粒体凋亡通路中的作用。通过敲除实验和免疫共沉淀发现,TMEM214与Bcl-2家族蛋白互作,调控细胞凋亡。抗体特异性通过siRNA敲低后的Western blot验证。
---
**备注**:上述文献为示例,实际文献需通过PubMed、Google Scholar或抗体公司官网(如CST、Abcam)查询。部分公司(如Sigma-Aldrich)的TMEM214抗体产品页面可能提供相关引用文献。
×