| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Methyltransferase-like protein 16, 211-, Methyltransferase 10 domain-containing protein, METTL16, METT10D |
| Entrez GeneID | 79066 |
| WB Predicted band size | 63.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MET10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of human MET10. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **MET10 (C-term) 抗体** 的3篇参考文献示例(注：以下内容为模拟生成，实际文献可能需要根据具体研究方向检索)：
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1. **文献名称**: *"Characterization of a polyclonal antibody against the C-terminal domain of MET10 in Saccharomyces cerevisiae"*
**作者**: Smith A, et al.
**摘要**: 本文报道了一种针对酵母MET10蛋白C端结构域的多克隆抗体的开发与验证。通过Western blot和免疫荧光实验证实该抗体可特异性识别MET10.并用于研究其在亚硫酸盐代谢通路中的亚细胞定位及表达水平变化。
2. **文献名称**: *"MET10 C-terminal antibody reveals dynamic localization during oxidative stress response"*
**作者**: Chen L, et al.
**摘要**: 研究利用MET10 (C-term)抗体探究酵母在氧化应激条件下MET10蛋白的细胞定位变化，发现其从细胞质向线粒体的迁移与硫代谢调控密切相关，提示MET10在应激响应中的新功能。
3. **文献名称**: *"Validation of a commercial MET10 C-terminal antibody for diagnostic applications in fungal pathogens"*
**作者**: Gomez-Rodriguez R, et al.
**摘要**: 评估了一种商业化MET10 C端抗体在致病真菌(如白色念珠菌)中的交叉反应性，证实其可用于临床样本中MET10蛋白的免疫组化检测，为真菌感染的分子诊断提供工具。
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**注**：以上文献为示例，实际研究中请通过PubMed或Web of Science以关键词“MET10 antibody C-terminal”检索最新文章。若MET10为特定研究模型中的蛋白，需结合具体物种或研究领域调整检索策略。
The MET10 (C-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the C-terminal region of the MET10 protein, a member of the methionine adenosyltransferase (MAT) family. MET10. also known as MAT2A, encodes the α2 catalytic subunit of MAT, an enzyme critical for synthesizing S-adenosylmethionine (SAMe), a universal methyl donor involved in methylation reactions, polyamine synthesis, and redox regulation. The C-terminal domain of MET10 is essential for enzymatic activity, substrate binding, and interaction with regulatory subunits (e.g., MAT2β).
This antibody is commonly used in research to study MET10 expression, localization, and function in cellular processes such as proliferation, differentiation, and metabolism. It has applications in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect MET10 in tissues or cell lines, particularly in contexts like liver disease, cancer, and neurological disorders where SAMe metabolism is dysregulated. The C-terminal specificity ensures recognition of full-length, functional MET10. avoiding cross-reactivity with truncated isoforms or related MAT family members (e.g., MAT1A).
Validation often includes testing in MET10-knockout models or siRNA-treated cells to confirm specificity. Researchers use this antibody to explore MET10's role in epigenetic regulation, oxidative stress responses, and its potential as a therapeutic target. Commercial versions are typically raised in rabbits or mice, with reactivity across human, mouse, and rat samples, and are cited in studies linking MAT2A dysfunction to hepatocellular carcinoma and Alzheimer’s disease.
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