| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rho guanine nucleotide exchange factor 3, Exchange factor found in platelets and leukemic and neuronal tissues, XPLN, ARHGEF3 |
| Entrez GeneID | 50650 |
| WB Predicted band size | 59.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ARHGEF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human ARHGEF3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与ARHGEF3抗体相关的文献概览：
1. **文献名称**：ARHGEF3 regulates skeletal remodeling and links RhoA signaling to osteoporosis
**作者**：Wu JY et al.
**摘要**：该研究利用ARHGEF3特异性抗体在小鼠模型中验证了该蛋白通过激活RhoA信号通路调控破骨细胞分化，并发现其表达异常与骨密度下降相关。抗体被用于Western blot和免疫组化分析骨组织样本。
2. **文献名称**：Genetic variation in ARHGEF3 impacts platelet activation and cardiovascular risk
**作者**：Johnson AD et al.
**摘要**：通过ARHGEF3抗体检测人血小板中的蛋白表达水平，结合GWAS数据发现其单核苷酸多态性(SNP)与血小板过度活化和血栓形成风险升高相关。研究强调了ARHGEF3在Rho GTP酶介导的血小板信号传导中的作用。
3. **文献名称**：ARHGEF3 promotes tumor invasion via RhoGTPase-dependent matrix metalloproteinase secretion
**作者**：Chen L et al.
**摘要**：在乳腺癌细胞系中，使用ARHGEF3抗体进行免疫荧光染色和Co-IP实验，证明该蛋白通过激活RhoA/ROCK通路增强MMP2/9分泌，促进肿瘤细胞侵袭。研究为ARHGEF3的促癌机制提供了实验证据。
(注：以上文献为示例性内容，实际文献需通过PubMed/Google Scholar检索关键词如"ARHGEF3 antibody"+"RhoGEF"获取近年研究。)
ARHGEF3 (Rho Guanine Nucleotide Exchange Factor 3) is a member of the RhoGEF family, which regulates Rho GTPases by catalyzing the exchange of GDP for GTP. It plays a key role in signaling pathways that modulate cytoskeletal dynamics, cell adhesion, and motility. Primarily expressed in osteoclasts, platelets, and certain immune cells, ARHGEF3 is implicated in bone metabolism, platelet activation, and inflammatory responses. Studies link ARHGEF3 dysregulation to osteoporosis, cardiovascular diseases, and cancer metastasis, highlighting its therapeutic and diagnostic potential.
ARHGEF3 antibodies are essential tools for detecting and studying the protein’s expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. High-quality ARHGEF3 antibodies are validated for specificity, often using knockout cell lines or tissues to confirm minimal cross-reactivity. Polyclonal antibodies offer broad epitope recognition, useful for detecting denatured proteins, while monoclonal antibodies provide consistent specificity for native conformations. Commercial ARHGEF3 antibodies often include validation data across multiple applications, ensuring reliability in diverse experimental models. Researchers utilize these antibodies to explore ARHGEF3’s role in Rho GTPase signaling pathways, disease mechanisms, and potential therapeutic targets.
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