| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E3 ubiquitin-protein ligase TRIM50, 632-, Tripartite motif-containing protein 50, TRIM50, TRIM50A |
| Entrez GeneID | 135892 |
| WB Predicted band size | 54.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TRIM50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-383 amino acids from the C-terminal region of human TRIM50. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TRIM50抗体的示例参考文献(内容为虚构示例，仅供格式参考)：
1. **文献名称**：*Development and Validation of a High-Specificity TRIM50 Antibody for Immunohistochemical Applications*
**作者**：Smith A et al.
**摘要**：本研究开发了一种针对TRIM50蛋白的新型兔多克隆抗体，并通过Western blot和免疫组化验证其特异性。该抗体成功应用于检测人类脑组织中TRIM50的表达模式，为神经退行性疾病研究提供了工具。
2. **文献名称**：*TRIM50 Antibody Reveals Its Role in Autophagy Regulation in Cancer Cell Lines*
**作者**：Chen L et al.
**摘要**：利用TRIM50特异性抗体，作者发现TRIM50通过调控自噬相关蛋白LC3的泛素化影响结肠癌细胞存活。抗体在小鼠模型中证实了TRIM50表达与肿瘤进展的负相关性。
3. **文献名称**：*A Monoclonal TRIM50 Antibody for Quantitative Proteomic Analysis*
**作者**：Gomez-Ruiz M et al.
**摘要**：本文报道了一种小鼠单克隆TRIM50抗体的制备，结合质谱技术定量分析了不同组织中TRIM50的表达水平，揭示了其在肝脏代谢中的潜在功能。
4. **文献名称**：*TRIM50 Antibody Identifies Novel Splice Variants in Neurodevelopmental Disorders*
**作者**：Wang X et al.
**摘要**：通过高灵敏度的TRIM50抗体，研究团队在自闭症患者脑样本中发现了TRIM50的两种新剪接变体，提示其可能参与突触可塑性调控。
(注：以上文献为模拟内容，实际引用需查询PubMed等数据库。)
The TRIM50 antibody is a research tool designed to detect and analyze TRIM50. a member of the tripartite motif (TRIM) protein family. TRIM proteins are characterized by conserved RING, B-box, and coiled-coil domains, often functioning as E3 ubiquitin ligases involved in diverse cellular processes, including protein degradation, innate immunity, and transcriptional regulation. TRIM50. encoded on chromosome 7 in humans, has been implicated in neurodevelopmental disorders, neurodegenerative diseases, and cancer, though its precise biological roles remain under investigation. Studies suggest it may regulate protein ubiquitination, autophagy, or neuronal differentiation pathways.
The TRIM50 antibody is typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. It enables researchers to study TRIM50 expression, localization, and interactions via techniques such as Western blotting, immunohistochemistry, or immunoprecipitation. Validation often includes testing in cell lines or tissues with confirmed TRIM50 expression and knockout controls to ensure specificity. Dysregulation of TRIM50 has been observed in conditions like Alzheimer’s disease and certain cancers, making this antibody valuable for exploring its pathological mechanisms. Researchers also use it to investigate TRIM50's role in cellular stress responses or viral defense, aligning with broader TRIM family functions. Proper validation remains critical due to potential cross-reactivity with homologous TRIM proteins.
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