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Rabbit Polyclonal EHMT2(N-term) Antibody

  • 中文名: EHMT2 (N-term)抗体
  • 别    名: Histone-lysine N-methyltransferase EHMT2, 211-, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, Protein G9a, EHMT2, BAT8, C6orf30, G9A, KMT1
货号: IPDX30696
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHistone-lysine N-methyltransferase EHMT2, 211-, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, Protein G9a, EHMT2, BAT8, C6orf30, G9A, KMT1C, NG36
Entrez GeneID10919
WB Predicted band size132.4kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis EHMT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-186 amino acids from the N-terminal region of human EHMT2.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于EHMT2(N-term)抗体的3篇参考文献示例,包含文献名称、作者及摘要内容概括:

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1. **"Selective Inhibition of EHMT2/G9a by a Small Molecule Inhibitor and Its Functional Consequences in Cancer Cells"**

*作者:Liu et al. (2020)*

**摘要**:本研究开发了一种特异性靶向EHMT2 N端结构域的小分子抑制剂,并利用N端抗体验证其在乳腺癌细胞中的效果。通过Western blot和免疫荧光,证实抑制剂可降低H3K9me2水平并抑制肿瘤生长。

2. **"Characterization of EHMT2 N-Terminal Antibody for Chromatin Immunoprecipitation (ChIP) Applications"**

*作者:Zhang et al. (2018)*

**摘要**:该文献系统验证了EHMT2 N端抗体在ChIP实验中的特异性,证明其能有效富集EHMT2结合的基因组区域(如SNAIL等基因启动子),并揭示其在表观遗传调控中的关键作用。

3. **"EHMT2/G9a Regulates Pluripotency in Embryonic Stem Cells via Histone Methylation"**

*作者:Tachibana et al. (2008)*

**摘要**:研究通过N端特异性抗体证实EHMT2在小鼠胚胎干细胞中调控多能性基因(如Oct4)的表达,并揭示其缺失导致H3K9me2减少及分化异常,强调其在维持干细胞状态中的作用。

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**说明**:以上文献为示例,实际引用需根据具体研究内容核实。建议通过PubMed或Google Scholar以关键词“EHMT2 N-term antibody”“G9a antibody validation”等检索最新研究。

背景信息

The EHMT2 (N-term) antibody targets the N-terminal region of EHMT2 (Euchromatic Histone Lysine Methyltransferase 2), also known as G9a, a key enzyme in epigenetic regulation. EHMT2 is a histone methyltransferase that primarily catalyzes mono- and dimethylation of histone H3 lysine 9 (H3K9me1/me2), contributing to transcriptional repression and heterochromatin formation. It forms a heterodimeric complex with EHMT1 (GLP) to mediate gene silencing, playing critical roles in cell differentiation, development, and X-chromosome inactivation. Dysregulation of EHMT2 is implicated in cancer, neurodevelopmental disorders, and metabolic diseases. The N-terminal-specific antibody is widely used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to study EHMT2 expression, localization, and interactions. Its specificity for the N-terminal region ensures detection of full-length EHMT2. avoiding cross-reactivity with truncated isoforms or homologous proteins. Researchers employ this antibody to explore EHMT2's role in chromatin remodeling, disease mechanisms, and therapeutic targeting, particularly in oncology and epigenetics. Validation typically includes knockout/knockdown controls to confirm specificity, ensuring reliable results in both basic and translational studies.

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