| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein-cysteine N-palmitoyltransferase HHAT, 231-, Hedgehog acyltransferase, Melanoma antigen recognized by T-cells 2, MART-2, Skinny hedgehog protein 1, HHAT, MART2, SKI1 |
| Entrez GeneID | 55733 |
| WB Predicted band size | 57.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-59 amino acids from the N-terminal region of human HHAT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HHAT(N-terminal)抗体的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"HHAT regulates the secretion of Sonic Hedgehog via a novel lysosomal pathway"*
**作者**：Smith A, et al.
**摘要**：本研究揭示了HHAT通过溶酶体途径调控Sonic Hedgehog(Shh)分泌的机制。作者利用HHAT(N-term)抗体进行Western blot和免疫荧光实验，证实HHAT在细胞内的定位及其与溶酶体标记物的共定位，为HHAT功能研究提供关键工具。
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2. **文献名称**：*"Characterization of HHAT monoclonal antibodies for studying Hedgehog signaling in development"*
**作者**：Chen L, et al.
**摘要**：文章报道了一种特异性靶向HHAT N端表位的单克隆抗体的开发与验证。通过免疫沉淀和免疫组化实验，该抗体成功用于检测小鼠胚胎组织中HHAT的表达模式，支持其在发育生物学中的应用。
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3. **文献名称**：*"HHAT inhibition suppresses tumor growth by disrupting Hedgehog pathway activity"*
**作者**：Wang Y, et al.
**摘要**：研究通过HHAT(N-term)抗体检测多种癌细胞系中HHAT的蛋白水平，发现抑制HHAT可降低Hedgehog信号活性并抑制肿瘤生长，为癌症靶向治疗提供潜在策略。
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注：以上文献为示例，实际引用时需根据具体研究补充真实发表的文献信息。
The HHAT (N-terminal) antibody is a tool used to detect the N-terminal region of Hedgehog acyltransferase (HHAT), an endoplasmic reticulum-resident enzyme critical for post-translational modification of Hedgehog (Hh) signaling proteins. HHAT catalyzes the attachment of a palmitate group to the N-terminal cysteine of Hh ligands (e.g., Sonic, Indian, and Desert Hedgehog), a process essential for their maturation, secretion, and functional activation. Dysregulation of HHAT has been implicated in developmental disorders and cancers, particularly those driven by aberrant Hh signaling, such as basal cell carcinoma and medulloblastoma.
This antibody, raised against a synthetic peptide corresponding to the human HHAT N-terminus, is commonly used in applications like Western blotting, immunoprecipitation, and immunofluorescence to study endogenous HHAT expression, localization, and regulation. Its specificity is often validated using HHAT-knockout cell lines or tissues. Researchers employ this reagent to explore HHAT's role in Hh-dependent processes, including embryogenesis, tissue homeostasis, and tumorigenesis. Additionally, it aids in evaluating HHAT as a therapeutic target in preclinical models. Optimal dilution ratios and experimental conditions may vary depending on sample type (e.g., cell lysates, frozen tissues). Commercial sources typically provide validation data across multiple species, with human and mouse reactivity most frequently reported.
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