| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD repeat-containing protein 43, WDR43, KIAA0007, UTP5 |
| Entrez GeneID | 23160 |
| WB Predicted band size | 74.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDR43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-665 amino acids from the C-terminal region of human WDR43. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDR43抗体的3篇参考文献的简要信息(注：以下内容基于假设性文献，实际文献需通过学术数据库验证)：
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1. **文献名称**: "Characterization of WDR43 in RNA Polymerase II Complex Assembly"
**作者**: Tanaka, R. et al.
**摘要**: 本研究利用特异性WDR43抗体，通过免疫共沉淀和质谱分析，揭示了WDR43作为RNA聚合酶II复合体的调控因子，参与转录延伸和mRNA加工的过程。抗体验证实验显示其在高通量蛋白质组学研究中的有效性。
2. **文献名称**: "WDR43 Antibody Development for Ciliopathy Research"
**作者**: Chen, M. & Liu, X.
**摘要**: 研究团队开发了一种兔源多克隆WDR43抗体，并验证其在免疫荧光和Western blot中的应用。结果显示，WDR43定位于纤毛基体，可能通过调控IFT(纤毛内运输)蛋白参与纤毛相关疾病的病理机制。
3. **文献名称**: "Functional Analysis of WDR43 in Zebrafish Neural Development"
**作者**: Gupta, S. et al.
**摘要**: 通过CRISPR/Cas9敲除结合WDR43抗体标记，研究发现WDR43缺失导致斑马鱼神经管发育异常。抗体染色结果显示，WDR43在神经前体细胞中高表达，提示其在神经分化中的潜在作用。
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**备注**：若需真实文献，建议通过PubMed或Google Scholar以“WDR43 antibody”或“WDR43 function”为关键词检索，并筛选包含抗体开发或应用的论文。
**Background of WDR43 Antibody**
WDR43 (WD Repeat Domain 43) is a protein encoded by the *WDR43* gene in humans, belonging to the WD-repeat protein family. These proteins are characterized by tandem repeats of conserved WD motifs, which facilitate protein-protein interactions and participate in diverse cellular processes. WDR43 is implicated in RNA metabolism, particularly in the biogenesis and processing of small nucleolar RNAs (snoRNAs) and ribosomal RNA (rRNA). It interacts with components of the U3 snoRNA complex, playing a role in ribosome assembly and pre-rRNA processing.
Studies suggest WDR43 localizes to the nucleolus and Cajal bodies, subcellular compartments critical for RNA modification and ribosome biogenesis. Dysregulation of WDR43 has been linked to developmental defects and diseases, including ciliopathies and cancers, due to its potential involvement in cell cycle regulation and cilia formation.
Antibodies targeting WDR43 are essential tools for investigating its expression, localization, and function. They are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to study WDR43's role in RNA processing pathways, nucleolar dynamics, and disease mechanisms. Recent research also explores its interaction with other proteins, such as TCOF1 (involved in Treacher Collins syndrome), highlighting its broader significance in cellular homeostasis and developmental biology.
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