| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Coiled-coil domain-containing protein 146, CCDC146, KIAA1505 |
| Entrez GeneID | 57639 |
| WB Predicted band size | 112.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CCDC146 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-141 amino acids from the N-terminal region of human CCDC146. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CCDC146(N-term)抗体的参考文献示例(注：以下内容为模拟虚构，实际文献可能需要通过学术数据库检索确认)：
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1. **文献名称**: *"Characterization of CCDC146 and its role in microtubule organization"*
**作者**: Smith A, et al. (2020)
**摘要**: 本研究通过生成兔源多克隆抗体(针对CCDC146的N端抗原表位)，揭示了该蛋白在细胞分裂过程中与微管结构的相互作用。抗体经免疫印迹和免疫荧光验证，证实其在HeLa细胞中特异性识别CCDC146.并发现其缺失导致纺锤体组装异常。
2. **文献名称**: *"CCDC146 interacts with ciliary proteins and its antibody reveals testis-specific expression"*
**作者**: Li X, et al. (2018)
**摘要**: 利用抗CCDC146 N端抗体进行组织染色，发现该蛋白在哺乳动物睾丸生精细胞中高表达，并与纤毛相关蛋白形成复合物。研究提示CCDC146可能在精子发生中调控纤毛功能，抗体特异性通过基因敲除实验验证。
3. **文献名称**: *"Development of a novel CCDC146 antibody for proteomic screening in lung cancer"*
**作者**: Chen H, et al. (2021)
**摘要**: 报道了一种高亲和力的CCDC146 N端单克隆抗体的开发，应用于肺癌组织芯片的免疫组化分析。结果显示CCDC146在非小细胞肺癌中过表达，且与患者预后相关，抗体特异性通过质谱和siRNA敲低实验确认。
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**备注**：上述文献为示例性质，实际研究中CCDC146相关抗体可能较少。建议通过以下途径获取准确信息：
1. 检索PubMed/Google Scholar关键词“CCDC146 antibody N-terminal”;
2. 查阅抗体供应商(如Abcam、Santa Cruz)的产品引用文献;
3. 关注涉及“卷曲螺旋蛋白功能”或“纤毛/精子发生相关研究”的论文。
The CCDC146 (N-term) antibody is designed to target the N-terminal region of the coiled-coil domain-containing protein 146 (CCDC146), a poorly characterized protein implicated in diverse cellular processes. CCDC146. encoded by the *CCDC146* gene, belongs to the family of coiled-coil domain proteins, which often participate in structural organization, intracellular transport, or signaling. While its exact biological function remains under investigation, CCDC146 is hypothesized to play roles in microtubule dynamics, cilia function, or gametogenesis, as suggested by its expression patterns in tissues like the testis and its interaction with proteins involved in cytoskeletal regulation.
The N-terminal-specific antibody enables researchers to study CCDC146’s expression, localization, and interactions in various experimental models (e.g., Western blot, immunofluorescence, immunohistochemistry). Its development often involves immunizing hosts with synthetic peptides or recombinant protein fragments corresponding to the N-terminal epitope, followed by affinity purification to ensure specificity. Validation typically includes verifying reactivity in knockout controls or confirming expected molecular weights (~100-120 kDa, depending on isoforms) and subcellular localization (e.g., cytoplasmic or perinuclear regions).
CCDC146 has drawn interest in reproductive biology due to its high expression in spermatogenic cells, suggesting a potential role in sperm maturation. Additionally, bioinformatic analyses link *CCDC146* mutations to ciliopathies or developmental disorders, though functional studies are ongoing. The antibody serves as a critical tool to unravel CCDC146’s contributions to these pathways and diseases.
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