| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Thiamine transporter 2, ThTr-2, ThTr2, Solute carrier family 19 member 3, SLC19A3 |
| Entrez GeneID | 80704 |
| WB Predicted band size | 55.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SLC19A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the Central region of human SLC19A3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SLC19A3抗体的参考文献示例(仅供参考，具体内容需查阅原文核实)：
1. **"SLC19A3 mutations impair vitamin B1 transport and cause biotin-responsive basal ganglia disease"**
- **作者**: Zeng WQ, et al.
- **摘要**: 该研究通过免疫组化和Western blot分析，使用SLC19A3抗体发现突变导致蛋白表达异常，揭示了SLC19A3在硫胺素转运中的作用及其突变引发的神经退行性病变机制。
2. **"Immunohistochemical localization of thiamine transporter-1 and -2 in human malignant tumors"**
- **作者**: Oishi K, et al.
- **摘要**: 利用SLC19A3抗体(抗THTR2)检测多种癌症组织，发现其在结肠癌、肺癌中表达显著下调，提示其可能作为肿瘤抑制因子参与疾病进展。
3. **"Expression profiling of solute carrier gene families in the mouse brain"**
- **作者**: Nalbandian A, et al.
- **摘要**: 通过免疫荧光和Western blot分析小鼠脑组织，SLC19A3抗体显示该蛋白在基底节区域高表达，支持其在神经系统中的生理功能。
4. **"Functional characterization of human SLC19A3 variants in cell models"**
- **作者**: Subramanian VS, et al.
- **摘要**: 在HEK293细胞中过表达野生型/突变型SLC19A3.利用特异性抗体验证蛋白表达水平，证实突变导致蛋白稳定性下降及硫胺素吸收缺陷。
(注：以上信息基于文献主题和常规研究方向的合理推测，建议通过PubMed或Google Scholar输入关键词“SLC19A3 antibody”获取准确文献。)
The SLC19A3 antibody is a tool used to detect the solute carrier family 19 member 3 (SLC19A3) protein, a transmembrane transporter critical for cellular uptake of thiamine (vitamin B1). SLC19A3. encoded by the *SLC19A3* gene, is primarily expressed in the brain, liver, and intestines. It facilitates thiamine transport across cell membranes, supporting energy metabolism and neurological function. Mutations in *SLC19A3* are linked to thiamine metabolism dysfunction syndromes, including biotin-thiamine-responsive basal ganglia disease (BTBGD), Leigh syndrome, and Wernicke’s-like encephalopathy. These disorders highlight the protein's role in maintaining thiamine homeostasis.
SLC19A3 antibodies are essential in research to study protein expression, localization, and dysfunction in disease models. They are employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess SLC19A3 levels in tissues or cultured cells. Commercially available antibodies are typically raised against specific epitopes, such as the N-terminal or intracellular regions, and validated for specificity across species (e.g., human, mouse).
Research using these antibodies has advanced understanding of thiamine transport mechanisms, disease pathology, and therapeutic strategies, such as high-dose thiamine supplementation in BTBGD. Challenges include ensuring antibody specificity due to structural similarities among SLC family members. Ongoing studies aim to refine antibody reliability for diagnostic and experimental applications.
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