| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytochrome c1, heme protein, mitochondrial, Complex III subunit 4, Complex III subunit IV, Cytochrome b-c1 complex subunit 4, Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit, Cytochrome c-1, CYC1 |
| Entrez GeneID | 1537 |
| WB Predicted band size | 35.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the C-terminal region of human CYC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CYC1抗体的3篇参考文献示例(注：以下内容为虚构示例，实际文献需通过学术数据库查询确认)：
1. **文献名称**：*Characterization of a Novel Polyclonal Antibody Against Human Cytochrome c1 for Mitochondrial Studies*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种针对人源细胞色素c1(CYC1)的多克隆抗体，通过Western blot和免疫荧光验证其特异性。实验表明该抗体适用于检测线粒体复合物III中CYC1的表达水平，为研究线粒体功能障碍相关疾病提供工具。
2. **文献名称**：*CYC1 Deficiency in Cardiac Tissue: Detection Using a Custom Monoclonal Antibody*
**作者**：Chen L, et al.
**摘要**：通过构建CYC1基因敲除小鼠模型，验证了一种单克隆抗体在心肌组织中的特异性。该抗体成功用于免疫组化分析，揭示了CYC1缺失与心脏氧化应激的相关性。
3. **文献名称**：*Role of Cytochrome c1 in Apoptosis: Insights from Antibody-Based Inhibition Assays*
**作者**：Wang Y, et al.
**摘要**：利用CYC1抗体阻断其在线粒体中的功能，研究发现CYC1在细胞凋亡过程中调控caspase激活的关键作用，为癌症治疗中的靶向策略提供依据。
如需具体文献，建议在PubMed或Web of Science中检索“Cytochrome c1 antibody”或“CYC1 antibody”结合研究领域(如“mitochondria”、“apoptosis”等)。
The CYC1 antibody targets cytochrome c1 (CYC1), a key subunit of mitochondrial Complex III (ubiquinol-cytochrome c reductase) in the electron transport chain (ETC). CYC1 facilitates electron transfer from ubiquinol to cytochrome c, critical for ATP synthesis via oxidative phosphorylation. Encoded by the nuclear *CYC1* gene, the protein is synthesized in the cytosol and imported into mitochondria, where it integrates into the inner mitochondrial membrane. Dysregulation of CYC1 has been linked to mitochondrial disorders, metabolic syndromes, and cancers, reflecting its role in cellular energy homeostasis.
CYC1 antibodies are widely used in research to investigate mitochondrial function, ETC assembly, and diseases associated with oxidative phosphorylation defects. They enable detection of CYC1 expression levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific applications include studying mitochondrial dynamics in neurodegeneration (e.g., Parkinson’s disease), cancer metabolism, and drug-induced mitochondrial toxicity. Many CYC1 antibodies are raised against conserved epitopes, ensuring cross-reactivity across human, mouse, and rat models. Validation typically involves knockdown experiments (siRNA/CRISPR) or mitochondrial fractionation to confirm specificity. Commercial antibodies often provide data on molecular weight (~34 kDa) and subcellular localization to mitochondria. Recent studies also utilize CYC1 antibodies to explore its non-canonical roles, such as apoptosis modulation through interactions with cytochrome c. Reliable CYC1 detection remains crucial for advancing mitochondrial biology and therapeutic targeting of ETC-related pathologies.
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