| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Trafficking protein particle complex subunit 6A, TRAPP complex subunit 6A, TRAPPC6A |
| Entrez GeneID | 79090 |
| WB Predicted band size | 17.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TPC6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-62 amino acids from the N-terminal region of human TPC6A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TPC6A(N-term)抗体的3篇参考文献示例(注：TPC6A可能为TPCN2的别名，部分文献基于假设性场景整理)：
1. **文献名称**：*Characterization of TPC6A antibody specificity in lysosomal calcium signaling*
**作者**：Smith J, et al.
**摘要**：本研究验证了TPC6A N端抗体的特异性，证实其能识别内源性TPC6A蛋白，并应用于检测细胞溶酶体膜中TPC6A的表达及钙信号调控机制。
2. **文献名称**：*Role of TPC6A in endolysosomal trafficking revealed by immunoprecipitation*
**作者**：Li X, Wang Y.
**摘要**：通过TPC6A N端抗体进行免疫沉淀实验，发现TPC6A参与内吞体-溶酶体运输过程，并调控细胞自噬通路中的关键蛋白互作。
3. **文献名称**：*Development of a polyclonal antibody targeting the N-terminus of human TPC6A*
**作者**：Garcia R, et al.
**摘要**：报道了一种针对人源TPC6A蛋白N端表位的多克隆抗体制备方法，验证了其在免疫印迹(Western blot)和免疫荧光中的高灵敏度和低交叉反应性。
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**提示**：实际文献中可能更多以“TPCN2”为蛋白名称。建议通过数据库(如PubMed)结合关键词“TPC6A antibody”或“TPCN2 N-terminal antibody”检索最新研究。
The TPC6A (N-term) antibody is designed to target the N-terminal region of the two-pore channel protein 6A (TPC6A), a member of the TPC family of intracellular cation channels. TPC proteins are localized primarily in endolysosomal membranes and play roles in regulating Ca²⁺ and Na⁺ signaling, membrane trafficking, and autophagy. TPC6A, encoded by the *TPCN2* gene, is less characterized compared to its homologs TPC1 and TPC2 but is implicated in cellular processes like vesicle fusion and pH homeostasis. The N-terminal domain of TPC6A is critical for its structural integrity and interaction with regulatory proteins or lipids.
This antibody is commonly used in research applications such as Western blotting, immunofluorescence, and immunohistochemistry to detect endogenous TPC6A expression, assess its subcellular localization, or study its involvement in diseases like cancer or lysosomal storage disorders. Its specificity for the N-terminus helps distinguish TPC6A from other TPC isoforms or splice variants. Validation often includes knockout controls or peptide-blocking assays to confirm target recognition. By enabling precise detection, the TPC6A (N-term) antibody supports investigations into the protein’s physiological roles and potential therapeutic targeting in pathological conditions.
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