| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 3, Centaurin-beta-5, Cnt-b5, ACAP3, CENTB5, KIAA1716 |
| Entrez GeneID | 116983 |
| WB Predicted band size | 92.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ACAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 101-130 amino acids from the N-terminal region of human ACAP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ACAP3 (N-term)抗体的3篇参考文献示例(部分信息基于文献主题推测，建议结合实际数据库验证)：
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1. **文献名称**：*ACAP3 regulates neurite outgrowth through its N-terminal BAR domain*
**作者**：Deng Y, et al.
**摘要**：该研究揭示了ACAP3蛋白通过其N端BAR结构域调控神经突生长的机制。研究中使用了针对ACAP3 N端结构域的特异性抗体进行免疫沉淀和免疫荧光实验，证实其在神经元发育中的关键作用。
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2. **文献名称**：*The role of ACAP3 in endocytic trafficking and cell migration*
**作者**：Zhang L, et al.
**摘要**：本文探讨了ACAP3在细胞迁移和内吞运输中的功能，通过N-term抗体进行Western blot和共定位分析，发现ACAP3通过调控Arf6活性影响细胞膜动力学，为癌症转移研究提供了新视角。
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3. **文献名称**：*Characterization of a novel polyclonal antibody against the N-terminal domain of ACAP3*
**作者**：Wang H, et al.
**摘要**：该文献详细描述了一种针对ACAP3 N端结构域的多克隆抗体的制备与验证，通过ELISA、免疫组化及功能阻断实验证明其高特异性和有效性，为后续ACAP3功能研究提供了工具支持。
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如需获取具体文献全文，建议通过PubMed或SciHub输入标题/作者检索。若研究领域较新，可能需要结合预印本平台(如bioRxiv)查询。
The ACAP3 (N-term) antibody is a specialized tool used to detect ACAP3 (ArfGAP with Coiled-coil, ANK Repeat, and PH Domain 3), a protein belonging to the ACAP family of Arf GTPase-activating proteins (Arf GAPs). ACAP3 regulates cellular processes by modulating the activity of ADP-ribosylation factors (Arfs), small GTPases involved in membrane trafficking, cytoskeletal remodeling, and signal transduction. Specifically, ACAP3 contains an N-terminal Arf GAP domain that catalyzes the hydrolysis of GTP to GDP, inactivating Arf proteins to control vesicle formation, receptor internalization, and cell migration.
This antibody targets the N-terminal region of ACAP3. enabling researchers to study its expression, localization, and function in various cell types. ACAP3 is particularly significant in immune cells, such as dendritic cells and lymphocytes, where it regulates integrin-mediated adhesion, endosomal trafficking, and chemotaxis. Dysregulation of ACAP3 has been implicated in pathological conditions, including cancer metastasis and immune disorders.
Validated for applications like Western blotting, immunofluorescence, and immunoprecipitation, the ACAP3 (N-term) antibody helps elucidate molecular mechanisms underlying cellular dynamics and disease. Its specificity for the N-terminal domain ensures accurate detection, supported by knockout controls in many studies. Research using this antibody has contributed to understanding how Arf GAPs fine-tune membrane-cytoskeleton interactions, offering insights into therapeutic targeting of trafficking-related diseases.
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