| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Forkhead box protein P3, Scurfin, FOXP3, IPEX |
| Entrez GeneID | 50943 |
| WB Predicted band size | 47.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FOXP3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S418 of human FOXP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是假设存在的关于Phospho-FOXP3(S418)抗体的参考文献示例(仅供格式参考)：
1. **文献名称**：*Phosphorylation of FOXP3 at Ser418 regulates its transcriptional activity in Treg cells*
**作者**：Chen Z. et al.
**摘要**：研究揭示了FOXP3在S418位点的磷酸化通过增强其与DNA结合能力，促进调节性T细胞(Treg)的免疫抑制功能，并利用Phospho-FOXP3(S418)抗体通过ChIP-seq验证其染色质定位变化。
2. **文献名称**：*Akt-mediated phosphorylation of FOXP3 at Ser418 modulates its stability in tumor microenvironment*
**作者**：Li H. et al.
**摘要**：报道Akt激酶介导的S418磷酸化保护FOXP3免受蛋白酶体降解，该抗体用于流式细胞术检测肿瘤浸润Treg中磷酸化水平与患者预后的关联。
3. **文献名称**：*Dysregulated FOXP3 phosphorylation at Ser418 contributes to autoimmune pathogenesis*
**作者**：Yamaguchi T. et al.
**摘要**：通过Phospho-FOXP3(S418)抗体发现，类风湿性关节炎患者Treg细胞中该位点磷酸化水平降低，导致其功能缺陷，提示其作为潜在治疗靶点。
**注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台以关键词“Phospho-FOXP3 S418”或“FOXP3 Ser418 phosphorylation”检索。
The Phospho-FOXP3(S418) antibody is a specialized tool used to detect the phosphorylation status of FOXP3. a critical transcription factor for regulatory T cells (Tregs). FOXP3 is essential for Treg development and function, playing a central role in maintaining immune tolerance and suppressing excessive immune responses. Post-translational modifications, including phosphorylation, regulate FOXP3's stability, localization, and transcriptional activity. The S418 phosphorylation site, located in the C-terminal region of FOXP3. has been implicated in modulating its interactions with co-factors or downstream targets, potentially influencing Treg suppressive capacity.
This antibody enables researchers to study FOXP3 activation dynamics in contexts like autoimmune diseases, cancer, and infections, where Treg dysfunction may contribute to pathogenesis. It is commonly used in techniques such as Western blotting, immunofluorescence, or flow cytometry to assess phosphorylation-dependent signaling pathways in T cells. Studies suggest that S418 phosphorylation may be regulated by kinases responsive to T-cell receptor (TCR) signaling or cytokine stimulation, linking environmental cues to FOXP3 functional states. However, the exact mechanistic role of this modification remains under investigation, with some evidence pointing to effects on protein-protein interactions or transcriptional complex assembly.
Validating this antibody's specificity is crucial, as cross-reactivity with other phosphorylated epitopes or FOXP3 isoforms could lead to misinterpretation. Its application advances understanding of immune regulation and therapeutic strategies targeting Treg activity.
×
