| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Roundabout homolog 4, Magic roundabout, ROBO4 |
| Entrez GeneID | 54538 |
| WB Predicted band size | 107.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **Roundabout 4 (ROBO4/N-term)** 抗体的示例参考文献(内容为模拟概括，建议通过学术数据库验证具体文献)：
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1. **文献名称**: *"ROBO4 regulates vascular stability and angiogenesis through selective binding to UNC5B"*
**作者**: Kuhnert F, et al.
**摘要**: 本研究利用针对ROBO4 N端的特异性抗体，揭示了ROBO4与受体UNC5B的相互作用在血管内皮细胞稳定性和病理性血管生成中的关键作用，为抗血管治疗提供了新靶点。
2. **文献名称**: *"Structural insights into the extracellular domain of ROBO4 and its role in endothelial cell signaling"*
**作者**: Huminiecki L, et al.
**摘要**: 通过N端特异性抗体阻断实验，结合结构生物学分析，阐明了ROBO4胞外结构域在调控内皮细胞迁移和血管屏障功能中的分子机制。
3. **文献名称**: *"ROBO4 expression in tumor vasculature: A biomarker for anti-angiogenic therapy resistance"*
**作者**: Jones CA, et al.
**摘要**: 使用ROBO4 N端抗体检测肿瘤血管中的表达水平，发现ROBO4高表达与抗VEGF治疗耐药性相关，提示其作为联合治疗靶点的潜力。
4. **文献名称**: *"ROBO4 modulates Wnt signaling in retinal angiogenesis"*
**作者**: Zhang B, et al.
**摘要**: 通过免疫组化和Western blot(N-term抗体验证)，证明ROBO4通过调控Wnt/β-catenin通路参与糖尿病视网膜病变中的异常血管生成。
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**注**：以上文献名称及内容为示例性概括，具体研究请通过 **PubMed/Google Scholar** 检索关键词如 *"ROBO4 antibody"*, *"ROBO4 N-terminal function"* 获取真实文献。
Roundabout 4 (Robo4), a member of the Roundabout (Robo) family of single-pass transmembrane receptors, plays a critical role in vascular development and endothelial cell signaling. Primarily expressed in vascular endothelial cells, Robo4 is involved in maintaining vascular integrity, regulating angiogenesis, and stabilizing blood vessels during development and pathological conditions. It interacts with Slit ligands and other guidance cues to modulate cell migration, adhesion, and barrier function. The N-terminal region of Robo4 is essential for ligand binding and receptor activation, making antibodies targeting this domain valuable tools for studying its functional mechanisms.
The Roundabout 4 (N-term) antibody is specifically designed to recognize the extracellular N-terminal epitope of Robo4. enabling applications such as Western blotting, immunohistochemistry, and immunofluorescence to detect Robo4 expression and localization. Research using this antibody has shed light on Robo4's role in vascular diseases, including diabetic retinopathy, tumor angiogenesis, and inflammatory disorders. By disrupting Robo4 signaling, studies explore its therapeutic potential in pathological angiogenesis or vascular leakage. The antibody's specificity for the N-terminal region ensures minimal cross-reactivity with other Robo family members, enhancing experimental accuracy. Overall, it serves as a crucial reagent for unraveling Robo4's contributions to vascular biology and disease.
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