| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Proteasomal ATPase-associated factor 1, Protein G-16, WD repeat-containing protein 71, PAAF1, WDR71 |
| Entrez GeneID | 80227 |
| WB Predicted band size | 42.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PAAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PAAF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PAAF1 (N-term)抗体的模拟参考文献示例(基于文献检索逻辑假设，实际文献可能需要进一步验证)：
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1. **文献名称**: *"PAAF1 (Proteasome Assembly Chaperone 1) regulates 26S proteasome biogenesis through binding to the N-terminal domain of PSMC5"*
**作者**: Tanaka K, et al.
**摘要**: 本研究揭示了PAAF1作为蛋白酶体组装伴侣的功能，通过其N端结构域与PSMC5的相互作用调控26S蛋白酶体的形成。研究中使用PAAF1 (N-term)抗体进行免疫共沉淀和Western blot实验，证实了其在蛋白酶体组装中的关键作用。
2. **文献名称**: *"Characterization of a novel monoclonal antibody targeting the N-terminus of PAAF1 for cancer biomarker studies"*
**作者**: Smith J, et al.
**摘要**: 文章报道了一种针对PAAF1 N端表位的新型单克隆抗体的开发与验证。通过免疫组化和流式细胞术，该抗体被证明在多种癌症组织中高特异性识别PAAF1.并可能作为肿瘤诊断的潜在标志物。
3. **文献名称**: *"PAAF1 interacts with BRCA1 to maintain genomic stability via the ubiquitin-proteasome pathway"*
**作者**: Chen L, et al.
**摘要**: 研究发现PAAF1通过N端结构域与BRCA1结合，参与DNA损伤修复。实验中使用PAAF1 (N-term)抗体进行染色质免疫沉淀(ChIP)和免疫荧光定位，证明其在维持基因组稳定性中的功能。
4. **文献名称**: *"A functional analysis of PAAF1 in neurodegenerative disease models using N-terminal specific antibodies"*
**作者**: Müller R, et al.
**摘要**: 本研究利用PAAF1 (N-term)抗体在小鼠脑组织中检测PAAF1的表达变化，发现其在阿尔茨海默病模型中表达下调，提示PAAF1可能通过调控蛋白酶体功能影响神经退行性病变进程。
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**注意**：以上为模拟文献，实际引用需查询PubMed、Google Scholar或抗体供应商(如Thermo Fisher、Abcam)提供的产品引用文献。建议通过关键词“PAAF1 antibody N-terminal”或“POMP antibody”结合具体研究领域进一步检索。
The PAAF1 (N-term) antibody targets the N-terminal region of Proteasomal ATPase-Associated Factor 1 (PAAF1), also known as PSMC3IP (HGNC:17919). PAAF1 is a nuclear protein implicated in proteasome function, DNA repair, and transcriptional regulation. It interacts with the 26S proteasome ATPase subunit PSMC3. modulating proteasomal activity involved in protein degradation. Additionally, PAAF1 plays a role in homologous recombination repair (HRR) by stabilizing RAD51 filaments during meiosis, critical for maintaining genomic stability. Its involvement in hormone-dependent transcription, particularly through interactions with estrogen receptors, links it to reproductive biology and cancer pathways.
The antibody is commonly used in research to study PAAF1's expression, localization, and molecular interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. Studies focus on its dual roles in proteostasis and DNA repair, with implications in infertility (e.g., premature ovarian insufficiency) and cancer biology, where dysregulated PAAF1 may influence tumor progression or therapy resistance. The N-terminal specificity helps distinguish PAAF1 isoforms or cleavage products in experimental models. As a research tool, this antibody aids in elucidating PAAF1's contributions to cellular stress responses and disease mechanisms.
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