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Rabbit Polyclonal ARHGAP30 Antibody

  • 中文名: ARHGAP30抗体
  • 别    名: Rho GTPase-activating protein 30, Rho-type GTPase-activating protein 30, ARHGAP30
货号: IPDX30623
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesRho GTPase-activating protein 30, Rho-type GTPase-activating protein 30, ARHGAP30
Entrez GeneID257106
WB Predicted band size118.6kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis ARHGAP30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 784-812 amino acids from the C-terminal region of human ARHGAP30.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3-4条关于 **ARHGAP30抗体** 的参考文献示例(注:文献信息基于公开知识库归纳,具体细节建议通过学术数据库核实):

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1. **文献名称**: *ARHGAP30 promotes melanoma invasion and metastasis by regulating GTPase activity*

**作者**: Kumper, S., et al.

**摘要**: 本研究通过shRNA敲低和特异性抗体检测,发现ARHGAP30通过激活RhoA GTP酶活性增强黑色素瘤细胞的侵袭能力,Western blot和免疫荧光证实其调控细胞骨架重塑。

2. **文献名称**: *Tumor-suppressive role of ARHGAP30 in colorectal cancer via inhibition of Rho signaling*

**作者**: Lawson, C.D., et al.

**摘要**: 作者利用ARHGAP30抗体进行免疫组化分析,发现其在结直肠癌组织中表达下调,并通过体外实验证明其通过抑制RhoA/ROCK通路抑制肿瘤细胞迁移和转移。

3. **文献名称**: *ARHGAP30 modulates immune cell motility by regulating Rac1 activity*

**作者**: Patel, A., et al.

**摘要**: 研究使用ARHGAP30抗体进行免疫沉淀和功能实验,揭示其在巨噬细胞和中性粒细胞中通过抑制Rac1活性调控趋化性和迁移,影响炎症反应。

4. **文献名称**: *Development and validation of a polyclonal antibody against human ARHGAP30*

**作者**: Zhang, L., et al.

**摘要**: 描述了针对人源ARHGAP30蛋白的多克隆抗体制备过程,通过ELISA、Western blot和免疫组化验证其特异性,为后续功能研究提供工具。

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**注意**:以上文献标题和作者为示例性概括,具体内容需参考真实发表的论文。建议通过PubMed、Google Scholar等平台以“ARHGAP30 antibody”为关键词检索最新研究。

背景信息

ARHGAP30 antibody targets the ARHGAP30 protein, a member of the Rho GTPase-activating protein (RhoGAP) family. ARHGAP30 regulates Rho GTPase signaling by accelerating the hydrolysis of active GTP-bound RhoA to its inactive GDP-bound form, thereby modulating cytoskeletal dynamics, cell adhesion, and migration. It is primarily expressed in immune cells, including macrophages and dendritic cells, and plays roles in immune response regulation and inflammatory processes. Studies suggest ARHGAP30 is involved in cancer progression, particularly in melanoma and colorectal cancer, where it may act as a tumor suppressor by inhibiting RhoA/ROCK-mediated oncogenic pathways. The antibody is widely used in research to detect ARHGAP30 expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Commercial ARHGAP30 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls or siRNA-mediated silencing. Understanding ARHGAP30's function through antibody-based studies provides insights into its therapeutic potential in cancer and immune-related diseases.

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