| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD and tetratricopeptide repeats protein 1, WDTC1, KIAA1037 |
| Entrez GeneID | 23038 |
| WB Predicted band size | 75.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDTC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 487-516 amino acids from the C-terminal region of human WDTC1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDTC1抗体的3篇代表性文献的简要概括(文献信息为示例，非真实引用)：
1. **《WDTC1 regulates adipogenesis through modulation of PPARγ stability》**
- **作者**: Smith A, et al.
- **摘要**: 研究利用WDTC1特异性抗体，通过免疫沉淀和Western blot分析，发现WDTC1通过抑制PPARγ蛋白的泛素化降解，调控脂肪细胞分化，揭示其在肥胖相关代谢疾病中的潜在作用。
2. **《WDTC1 as a tumor suppressor in colorectal cancer: Antibody-based expression profiling》**
- **作者**: Lee J, et al.
- **摘要**: 通过免疫组化(WDTC1抗体)分析结直肠癌组织样本，发现WDTC1蛋白表达水平与肿瘤分期呈负相关，提示其可能通过调控Wnt/β-catenin通路抑制肿瘤进展。
3. **《Antibody screening identifies WDTC1 as a novel interactor of the Hedgehog signaling pathway》**
- **作者**: Zhang Y, et al.
- **摘要**: 采用Co-IP联合质谱技术(基于WDTC1抗体)，发现WDTC1与Hedgehog通路关键蛋白Gli1直接互作，可能通过表观遗传修饰调控靶基因转录，影响胚胎发育。
**注**：以上文献名为虚拟示例，实际研究中建议通过PubMed或Google Scholar以“WDTC1 antibody”为关键词检索最新文献。
The WDTC1 (WD and tetratricopeptide repeats 1) antibody is a tool used to study the WDTC1 protein, encoded by the *WDTC1* gene in humans. WDTC1. also known as Adipose (Adp), is a conserved regulatory component of the ubiquitin ligase complex that plays a role in lipid metabolism and energy homeostasis. Structurally, it contains WD40 and tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions. Research suggests WDTC1 acts as a suppressor of adipogenesis by promoting the ubiquitination and degradation of key lipid synthesis enzymes, thereby inhibiting excessive fat accumulation. Studies in *Drosophila* and mammals link WDTC1 dysfunction to obesity-related phenotypes, highlighting its potential relevance in metabolic disorders.
The WDTC1 antibody is widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and interaction partners in various tissues, particularly adipose tissue. It aids in exploring WDTC1's role in metabolic pathways, including its interplay with enzymes like ACC (acetyl-CoA carboxylase) and regulators of lipogenesis. Commercial WDTC1 antibodies are typically raised in rabbits or mice, validated for specificity using knockout controls or siRNA-mediated silencing. Its application extends to obesity, diabetes, and cancer research, where altered lipid metabolism is a key focus. Understanding WDTC1's mechanisms may offer insights into therapeutic strategies for metabolic diseases.
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