| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lysine-specific demethylase 4A, 11411-, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, KDM4A, JHDM3A, JMJD2, JMJD2A, KIAA0677 |
| Entrez GeneID | 9682 |
| WB Predicted band size | 120.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This JMJD2A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y547 of human JMJD2A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-JMJD2A(Y547)抗体的3篇参考文献示例(注：以下内容为基于领域知识的模拟示例，实际文献需通过数据库验证)：
1. **文献名称**：*Phosphorylation of JMJD2A at Tyrosine 547 Regulates Its Chromatin Binding and Demethylase Activity*
**作者**：Chen L, et al.
**摘要**：该研究发现JMJD2A(KDM4A)在Y547位点的磷酸化由EGFR激酶介导，磷酸化修饰增强了其与染色质的结合能力，并促进H3K9me3的去甲基化，进而影响癌细胞增殖。
2. **文献名称**：*DNA Damage-Induced JMJD2A Phosphorylation Modulates Its Role in DNA Repair*
**作者**：Wang Y, et al.
**摘要**：研究揭示了ATM激酶在DNA损伤后磷酸化JMJD2A的Y547位点，磷酸化修饰促进JMJD2A与53BP1蛋白的相互作用，从而调控同源重组修复(HR)通路。
3. **文献名称**：*Akt-Mediated Phosphorylation of JMJD2A at Y547 Promotes Tumor Metastasis*
**作者**：Kim S, et al.
**摘要**：本文证明Akt信号通路通过磷酸化JMJD2A的Y547位点，抑制其核输出并增强上皮间质转化(EMT)相关基因表达，最终促进乳腺癌转移。
**注意事项**：
- 若实际文献不足，可能与Y547位点研究较少或命名差异有关，建议扩展检索关键词(如KDM4A、H3K9me3去甲基化、酪氨酸磷酸化)。
- 部分商业抗体的产品说明书可能引用相关文献(如CST或Abcam的抗体目录)。
**Background of Phospho-JMJD2A (Y547) Antibody**
JMJD2A (also known as KDM4A) is a histone demethylase that specifically targets tri-/dimethylated lysine residues on histones H3K9 and H3K36. playing a critical role in epigenetic regulation of gene expression and chromatin remodeling. Phosphorylation at tyrosine 547 (Y547) is a post-translational modification implicated in modulating JMJD2A's enzymatic activity, subcellular localization, or interaction with regulatory partners. This modification may influence its role in cellular processes such as DNA repair, cell cycle progression, and transcriptional regulation.
The Phospho-JMJD2A (Y547) antibody is a specialized tool designed to detect JMJD2A specifically phosphorylated at this residue. It is commonly used in research to study signaling pathways involving tyrosine kinases or phosphatases that regulate JMJD2A function. Studies utilizing this antibody have explored its relevance in cancer biology, as dysregulation of JMJD2A is linked to tumorigenesis, metastasis, and therapeutic resistance. Validation of the antibody typically includes Western blotting, immunofluorescence, or immunohistochemistry to ensure specificity for the phosphorylated epitope.
Understanding JMJD2A phosphorylation dynamics provides insights into how epigenetic modifiers integrate cellular signals to control chromatin states, offering potential therapeutic targets for diseases driven by epigenetic dysregulation.
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