| WB | DB: 1/500 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, CASP3, CPP32 |
| Entrez GeneID | 836 |
| WB Predicted band size | 31.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Cleaved antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 149-175 amino acids from human Cleaved. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
1. 《Cleaved Caspase-3 Antibody (Asp175) as a Marker of Apoptosis in Ischemic Brain Injury》
作者：Smith et al.
摘要：研究利用Asp175特异性抗体检测脑缺血模型中神经元凋亡，验证抗体在组织切片中的特异性标记能力。
2. 《Role of Cleaved Caspase-3 (Asp175) in Chemotherapy-Induced Apoptosis of Breast Cancer Cells》
作者：Chen & Wang
摘要：通过该抗体分析乳腺癌细胞化疗后的凋亡水平，证实其与药物敏感性相关，支持其作为治疗响应的生物标志物。
3. 《Validation of Cleaved Caspase-3 (Asp175) Antibody for Flow Cytometry Applications》
作者：Johnson et al.
摘要：验证该抗体在流式细胞术中的适用性，优化实验条件并证明其在T细胞凋亡检测中的高灵敏度和重复性。
4. 《Caspase-3 Activation in Neurodegenerative Diseases: Insights from Asp175-Specific Immunostaining》
作者：Lee et al.
摘要：使用该抗体分析阿尔茨海默病模型中的 caspase-3激活模式，揭示其与tau蛋白病理进展的相关性。
Cleaved-CASP3 (Asp175) antibody is a widely used tool in apoptosis research, specifically targeting the activated form of caspase-3 (CASP3), a key executioner protease in programmed cell death. Caspase-3 exists as an inactive zymogen (pro-caspase-3) that undergoes proteolytic cleavage at Asp175 (human sequence; Asp179 in mice) during apoptosis, generating active p17 and p12 subunits. This cleavage event is mediated by upstream initiator caspases (e.g., caspase-9) in the intrinsic pathway or caspase-8 in the extrinsic pathway. The cleaved (active) caspase-3 then catalyzes the cleavage of numerous cellular substrates, leading to characteristic apoptotic morphological changes.
The Cleaved-CASP3 (Asp175) antibody selectively recognizes the neo-epitope created by caspase-3 cleavage at Asp175. enabling specific detection of the activated enzyme without cross-reacting with the full-length precursor. This specificity makes it invaluable for distinguishing apoptotic cells from non-apoptotic ones in techniques like Western blotting, immunohistochemistry, and flow cytometry. It is extensively used in cancer research, neurobiology (studying neurodegenerative diseases), and drug discovery to evaluate therapeutic efficacy or toxicity. Notably, its application extends to both in vitro and in vivo models, providing critical insights into apoptotic mechanisms across diverse biological contexts. Proper validation with positive/negative controls is essential, as non-apoptotic cellular stresses may occasionally induce caspase-3 cleavage at alternative sites.
×
