| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase, GlcNAc-1-P transferase, G1PT, GPT, N-acetylglucosamine-1-phosphate transferase, DPAGT1, DPAGT2 |
| Entrez GeneID | 1798 |
| WB Predicted band size | 46.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DPAGT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human DPAGT1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DPAGT1抗体的3篇参考文献及其摘要概括：
1. **"DPAGT1 mutations cause a congenital disorder of glycosylation with defective α-dystroglycan glycosylation"**
- **作者**: Wu X, et al.
- **摘要**: 本研究通过基因分析发现DPAGT1基因突变导致先天性糖基化障碍(CDG-Ij)，并利用DPAGT1特异性抗体检测患者细胞中酶的表达水平，发现其功能缺失引发α-dystroglycan糖基化异常，进而导致肌肉和神经系统病变。
2. **"A novel monoclonal antibody reveals the conformational change of DPAGT1 during catalysis"**
- **作者**: Stals KL, et al.
- **摘要**: 研究团队开发了一种针对DPAGT1的单克隆抗体，通过免疫沉淀和免疫荧光技术证实该抗体可特异性识别DPAGT1的活性构象，并揭示其在催化过程中因底物结合发生的构象变化，为疾病诊断提供工具。
3. **"Aberrant DPAGT1 expression in lung cancer cells detected by immunohistochemistry"**
- **作者**: Wang Y, et al.
- **摘要**: 采用DPAGT1抗体对肺癌组织进行免疫组化分析，发现DPAGT1在肿瘤细胞中显著高表达，且与患者预后不良相关，提示其可能作为癌症治疗的潜在靶点。
*注：以上文献信息为模拟示例，实际文献需通过学术数据库(如PubMed)检索确认。*
DPAGT1 (Dolichyl-phosphate N-acetylglucosaminephosphotransferase 1) is a key enzyme in the N-linked glycosylation pathway, catalyzing the initial step of the dolichol cycle in the endoplasmic reticulum. This enzyme transfers N-acetylglucosamine-1-phosphate from UDP-GlcNAc to dolichyl phosphate, forming GlcNAc-pyrophosphoryldolichol—a critical precursor for synthesizing lipid-linked oligosaccharides (LLOs) required for protein glycosylation. Glycosylation mediated by DPAGT1 is essential for protein folding, cell adhesion, and signaling, impacting cellular homeostasis and development.
DPAGT1 antibodies are immunological tools designed to detect and study the expression, localization, and function of this enzyme. These antibodies are widely used in research applications such as Western blotting, immunofluorescence, and immunoprecipitation to investigate glycosylation-related mechanisms in diseases. Mutations in the DPAGT1 gene are linked to congenital disorders of glycosylation (CDGs), notably DPAGT1-CDG, characterized by neuromuscular defects and developmental delays. Additionally, dysregulated DPAGT1 expression is implicated in cancer progression, diabetes, and Alzheimer’s disease, making its antibodies valuable for exploring pathological glycosylation.
Commercially available DPAGT1 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein. Validation often includes knockout cell line controls to confirm specificity. Researchers utilize these antibodies to elucidate DPAGT1’s role in endoplasmic reticulum stress, metabolic regulation, and therapeutic targeting, particularly in cancers where glycosylation pathways are aberrantly activated.
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