| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Non-structural maintenance of chromosomes element 4 homolog A, NS4EA, Non-SMC element 4 homolog A, NSMCE4A, C10orf86 |
| Entrez GeneID | 54780 |
| WB Predicted band size | 44.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NSE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human NSE4A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于NSE4A(N-term)抗体的3篇参考文献的简要概述(注：文献信息为模拟示例，实际引用需核实)：
---
1. **文献名称**：*STAG3/NSE4A is essential for meiotic chromosome cohesion in mammalian germ cells*
**作者**：Garcia-Cruz R. et al.
**摘要**：该研究利用针对STAG3(NSE4A)N端的特异性抗体，揭示了其在哺乳动物生殖细胞减数分裂中的关键作用，证实STAG3通过维持黏连蛋白复合体的稳定性保障染色体正确分离。
2. **文献名称**：*Structural basis of cohesin interaction with STAG3 in human cells*
**作者**：Haering C.H. et al.
**摘要**：通过NSE4A(STAG3)N端抗体的免疫共沉淀实验，解析了黏连蛋白复合体中STAG3与其他亚基(如SMC1/SMC3)的结合模式，阐明了其维持染色体结构的分子机制。
3. **文献名称**：*Dysregulation of STAG3 expression correlates with poor prognosis in testicular cancer*
**作者**：Li Y. et al.
**摘要**：研究采用NSE4A(STAG3)N端抗体进行免疫组化分析，发现STAG3在睾丸癌中异常低表达，提示其可能作为潜在生物标志物，与患者生存率显著相关。
---
**备注**：以上文献为示例性质，实际研究中请通过PubMed或Web of Science等平台检索具体文献，并结合实验需求选择经过验证的抗体相关研究。
The NSE4A (N-term) antibody is a research tool designed to target the N-terminal region of the Non-SMC Element 4A (NSE4A) protein, a critical component of the Structural Maintenance of Chromosomes (SMC) complex family. NSE4A, also known as NCAPG2 or Kleisin-γ, functions as a regulatory subunit within the condensin II complex, which plays a vital role in chromosome organization, condensation, and segregation during mitosis and meiosis.
Condensin II, composed of SMC2. SMC4. and non-SMC subunits like NSE4A, ensures proper chromatin architecture by introducing positive supercoils into DNA, facilitating chromosomal compaction. The N-terminal domain of NSE4A is essential for binding to SMC subunits and stabilizing the complex. Dysregulation of NSE4A is linked to genomic instability, mitotic errors, and diseases such as cancer, underscoring its importance in maintaining genome integrity.
The NSE4A (N-term) antibody is widely used in molecular biology to study condensin II dynamics, chromatin organization, and cell cycle regulation. Applications include Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Researchers utilize this antibody to investigate how NSE4A mutations or expression changes contribute to chromosomal abnormalities and disease pathogenesis, offering insights into potential therapeutic targets for cancer and developmental disorders. Its specificity for the N-terminal region ensures detection of full-length or truncated variants, aiding functional studies.
×
