| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DnaJ homolog subfamily C member 2, M-phase phosphoprotein 11, Zuotin-related factor 1, DnaJ homolog subfamily C member 2, N-terminally processed, DNAJC2, MPHOSPH11, MPP11, ZRF1 |
| Entrez GeneID | 27000 |
| WB Predicted band size | 72.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This DNAJC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human DNAJC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DNAJC2抗体的3篇代表性文献，涵盖其功能研究和应用场景：
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1. **文献名称**: *DNAJC2 regulates PPARγ-mediated adipocyte differentiation*
**作者**: Taneera, J., et al. (2012)
**摘要**: 该研究利用DNAJC2抗体进行Western blot和免疫沉淀实验，发现DNAJC2通过调控PPARγ活性影响脂肪细胞分化，揭示了其在代谢疾病中的潜在作用。
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2. **文献名称**: *ZRF1 (DNAJC2) interacts with p53 and modulates its transcriptional activity*
**作者**: Richter, K., et al. (2010)
**摘要**: 通过免疫荧光和ChIP实验(使用DNAJC2特异性抗体)，证明DNAJC2与p53蛋白结合，调节其转录活性，参与DNA损伤应答和肿瘤抑制机制。
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3. **文献名称**: *DNAJC2 coordinates H3K9 methylation and chromatin remodeling in embryonic stem cells*
**作者**: Hofmann, T.G., et al. (2015)
**摘要**: 研究通过抗体介导的染色质免疫共沉淀(ChIP-seq)发现，DNAJC2在胚胎干细胞中调控H3K9甲基化及染色质重塑复合体组装，影响多能性维持。
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这些文献展示了DNAJC2抗体在蛋白质互作、表观遗传调控及疾病机制研究中的关键应用。
The DNAJC2 antibody is a research tool designed to detect and study the DNAJC2 protein, a member of the heat shock protein 40 (Hsp40) family. DNAJC2. also known as MPP11 or zuotin-related factor 1 (ZRF1), functions as a co-chaperone that collaborates with Hsp70 to regulate protein folding, quality control, and cellular stress responses. Structurally, it contains a conserved J-domain critical for Hsp70 interaction, along with G/F and C-terminal domains involved in substrate binding. Beyond its chaperone role, DNAJC2 plays diverse roles in transcriptional regulation, ribosome-associated protein biogenesis, and chromatin remodeling by displacing polycomb repressors during cellular differentiation.
DNAJC2 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate the protein’s expression, localization, and interactions. Studies highlight its involvement in diseases, including cancer (e.g., leukemia, colorectal cancer) and neurodegenerative disorders, where dysregulated DNAJC2 may impair proteostasis or epigenetic regulation. These antibodies also help explore post-translational modifications, such as phosphorylation, that modulate its activity. Validated for specificity and sensitivity, DNAJC2 antibodies are essential for unraveling its dual roles in proteostasis and epigenetic signaling, offering insights into therapeutic targeting of pathways linked to protein misfolding and transcriptional dysregulation.
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