| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP |
| Entrez GeneID | 8329;8330;8332;8336;8969 |
| WB Predicted band size | 14.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIST1H2AL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the C-terminal region of human HIST1H2AL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **HIST1H2AL 抗体** 的参考文献示例(注：以下文献信息为虚构，仅用于示例)：
1. **标题**: "HIST1H2AL as a novel biomarker in colorectal cancer: Immunohistochemical analysis"
**作者**: Zhang Y, et al.
**摘要**: 研究使用 HIST1H2AL 抗体对结直肠癌组织进行免疫组化分析，发现其表达水平与肿瘤分期和患者生存率显著相关，提示其作为预后标志物的潜力。
2. **标题**: "Epigenetic regulation by histone H2A variants: Role of HIST1H2AL in chromatin remodeling"
**作者**: Müller S, et al.
**摘要**: 通过 ChIP-seq 和 Western blot(使用 HIST1H2AL 特异性抗体)，阐明 HIST1H2AL 在染色质重塑和基因沉默中的调控机制，尤其在干细胞分化中的作用。
3. **标题**: "Autoantibodies against HIST1H2AL in systemic lupus erythematosus"
**作者**: Gupta R, et al.
**摘要**: 发现系统性红斑狼疮(SLE)患者血清中存在抗 HIST1H2AL 的自身抗体，可能通过 ELISA 和蛋白质组学方法验证其与疾病活动的相关性。
4. **标题**: "Development of a monoclonal antibody targeting HIST1H2AL for epigenetic studies"
**作者**: Lee JH, et al.
**摘要**: 描述一种新型 HIST1H2AL 单克隆抗体的开发与验证，证明其在免疫荧光和流式细胞术中的高特异性，适用于表观遗传学研究。
(如需真实文献，建议通过 PubMed 或 Google Scholar 检索关键词 "HIST1H2AL antibody" 或联系相关领域数据库获取。)
The HIST1H2AL antibody targets a specific histone protein, HIST1H2AL, which belongs to the histone H2A family. Histones are essential nuclear proteins that organize DNA into nucleosomes, the fundamental units of chromatin. As a core component of nucleosomes, HIST1H2AL plays a critical role in maintaining chromatin structure and regulating gene expression through post-translational modifications (e.g., acetylation, methylation) that influence DNA accessibility. The HIST1H2AL gene is part of the HIST1 cluster on chromosome 6. which encodes multiple replication-dependent histone variants.
Antibodies against HIST1H2AL are widely used in epigenetics and molecular biology research to investigate histone modification patterns, chromatin remodeling, and gene regulation mechanisms. They are applied in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity is crucial due to high sequence similarity among H2A family members, so rigorous validation (e.g., knockout controls) is required.
Studies involving HIST1H2AL antibodies often focus on cellular processes like DNA repair, replication, and transcriptional regulation, as well as diseases linked to chromatin dysregulation, including cancers and developmental disorders. These tools help uncover how histone variants contribute to cellular identity and disease pathology.
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