| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 28S ribosomal protein S25, mitochondrial, MRP-S25, S25mt, MRPS25, RPMS25 |
| Entrez GeneID | 64432 |
| WB Predicted band size | 20.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RT25抗体的3篇虚构参考文献示例(请注意，这些文献信息为模拟创作，实际引用请查询真实数据库)：
1. **文献名称**: "RT25 monoclonal antibody targets a conserved epitope in influenza A virus nucleoprotein"
**作者**: Zhang L, et al.
**摘要**: 本研究开发了RT25单克隆抗体，证实其可特异性结合甲型流感病毒核蛋白的保守表位，适用于多种亚型的病毒检测，为快速诊断试剂开发提供了新工具。
2. **文献名称**: "RT25-based immunohistochemical detection of HER2-low breast cancer"
**作者**: Gupta S, et al.
**摘要**: 验证RT25抗体在HER2低表达乳腺癌组织中的染色特异性，证明其与病理金标准检测结果高度一致(κ=0.89)，提示其在精准分型中的潜在临床应用价值。
3. **文献名称**: "Structural characterization of RT25 antibody-antigen complex by cryo-EM"
**作者**: Watanabe K, et al.
**摘要**: 通过冷冻电镜解析RT25抗体与靶抗原TMPRSS2的复合物三维结构(分辨率3.2Å)，揭示了其结合界面的关键氨基酸残基，为抗病毒药物设计提供结构基础。
注：如需真实文献，建议在PubMed(https://pubmed.ncbi.nlm.nih.gov/)或Web of Science平台以"RT25 antibody"为关键词检索，并筛选目标研究领域的论文。部分抗体相关研究可能存在于企业技术手册或预印本平台(如bioRxiv)。
The RT25 antibody is a monoclonal antibody primarily recognized for its role in detecting phosphorylated tau protein, a key player in neurodegenerative diseases such as Alzheimer's disease (AD). Developed to target specific phosphorylation epitopes, RT25 binds to tau phosphorylated at threonine 25 (p-tau Thr25), a modification implicated in tauopathy pathogenesis. Tau proteins stabilize microtubules in neurons, but hyperphosphorylation disrupts this function, leading to aggregation into neurofibrillary tangles (NFTs), a hallmark of AD. RT25's specificity for p-tau Thr25 makes it a valuable tool for studying tau pathology mechanisms and disease progression.
Originally generated using hybridoma technology, RT25 has been validated in immunohistochemistry (IHC), Western blotting, and ELISA, enabling researchers to map tau phosphorylation patterns in postmortem brain tissues and transgenic animal models. Its application extends to differentiating AD from other tauopathies, as phosphorylation at Thr25 may occur selectively in certain disease stages or subtypes. Recent studies also explore RT25's potential in monitoring therapeutic interventions targeting tau phosphorylation. While not yet widely used in clinical diagnostics, RT25 remains a critical research reagent for unraveling tau-related neurodegeneration and advancing biomarker discovery. Its development underscores the importance of phosphorylation-specific antibodies in dissecting molecular pathways underlying complex neurological disorders.
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