| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ubiquitin thioesterase ZRANB1, TRAF-binding domain-containing protein, hTrabid, Zinc finger Ran-binding domain-containing protein 1, ZRANB1, TRABID |
| Entrez GeneID | 54764 |
| WB Predicted band size | 81.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ZRANB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 665-692 amino acids from the C-terminal region of human ZRANB1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZRANB1抗体的3篇文献示例(部分信息为模拟，仅供参考)：
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1. **文献名称**：*ZRANB1 is a novel binding partner of the E3 ubiquitin ligase TRIM25 and regulates its function in RNA virus signaling*
**作者**：Smith J, et al.
**摘要**：本研究通过免疫共沉淀(Co-IP)和Western blot技术，利用ZRANB1特异性抗体证实了ZRANB1与TRIM25蛋白的相互作用，揭示了其在抗病毒天然免疫通路中调控RIG-I泛素化的新机制。
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2. **文献名称**：*Structural and functional analysis of ZRANB1 in DNA damage repair*
**作者**：Lee H, et al.
**摘要**：作者通过ZRANB1抗体进行免疫荧光染色，发现ZRANB1在DNA损伤位点富集，并结合CRISPR敲除技术，证明其通过结合PCNA促进同源重组修复的功能。
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3. **文献名称**：*ZRANB1 promotes tumor metastasis via alternative splicing regulation in breast cancer*
**作者**：Wang Y, et al.
**摘要**：该研究使用ZRANB1抗体对乳腺癌组织样本进行免疫组化分析，发现ZRANB1高表达与患者预后不良相关，并通过RNA-seq发现其通过调控可变剪接驱动肿瘤转移。
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**注意**：以上文献为示例，实际引用时建议通过PubMed或Google Scholar以“ZRANB1 antibody”或“ZRANB1 function”等关键词检索最新研究。
ZRANB1 (zinc finger RANBP2-type containing 1), also known as TRABID, is a deubiquitinating enzyme (DUB) belonging to the ovarian tumor (OTU) domain protease family. It plays a regulatory role in diverse cellular processes, including DNA damage repair, cell cycle progression, and Wnt/β-catenin signaling. ZRANB1 recognizes and cleaves lysine 29 (K29)- and lysine 33 (K33)-linked polyubiquitin chains, modulating substrate protein stability and interaction networks. Structurally, it contains an N-terminal OTU domain critical for catalytic activity and multiple zinc finger motifs implicated in substrate recognition.
ZRANB1 antibodies are essential tools for studying its expression, localization, and molecular functions. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate ZRANB1's involvement in diseases like cancer and neurodegenerative disorders. For instance, ZRANB1 overexpression has been linked to tumor progression via dysregulation of Wnt signaling, while its interaction with VCP/p97 highlights potential roles in protein quality control. Antibodies targeting specific epitopes (e.g., N-terminal or OTU domain) help dissect structure-function relationships. Commercial ZRANB1 antibodies are typically validated in knockout cell lines to ensure specificity. Research on ZRANB1 continues to uncover its therapeutic potential, particularly in contexts of genomic instability and HECT-type E3 ligase regulation.
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