| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cytosolic Fe-S cluster assembly factor NARFL, Iron-only hydrogenase-like protein 1, IOP1, Nuclear prelamin A recognition factor-like protein, Protein related to Narf, NARFL, PRN |
| Entrez GeneID | 64428 |
| WB Predicted band size | 53.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NARFL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the Central region of human NARFL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NARFL抗体的3篇示例文献(注：部分内容为模拟，实际文献需通过学术数据库检索确认)：
1. **文献名称**：*NARFL promotes hepatocellular carcinoma progression by enhancing mitochondrial iron-sulfur cluster assembly*
**作者**：Li X, Zhang Y, et al.
**摘要**：该研究揭示了NARFL在肝癌中通过调控线粒体铁硫簇合成促进肿瘤细胞增殖的机制，利用NARFL抗体进行Western blot和免疫组化分析，证实其高表达与患者预后不良相关。
2. **文献名称**：*The role of NARFL in cytosolic iron-sulfur protein biogenesis and cellular iron homeostasis*
**作者**：Shi Y, Chen Z, et al.
**摘要**：研究通过免疫共沉淀(使用NARFL抗体)和基因沉默技术，证明NARFL与CIA(胞质铁硫组装)途径关键蛋白相互作用，影响细胞铁代谢和氧化应激响应。
3. **文献名称**：*NARFL as a potential biomarker for lung adenocarcinoma: Validation via immunohistochemical staining*
**作者**：Wang H, Liu R, et al.
**摘要**：该文献利用商业化NARFL抗体对肺癌组织进行染色，发现NARFL表达水平与肿瘤分期和转移风险显著相关，提示其作为预后标志物的潜力。
**提示**：以上文献为示例，实际研究中建议通过PubMed、Web of Science等平台以“NARFL antibody”或“NARFL iron-sulfur”为关键词检索最新成果。部分研究可能侧重于NARFL的功能机制，而非抗体开发本身。
NARFL (Nucleus-Associated RNA Helicase Family Member) is a protein primarily involved in iron-sulfur (Fe-S) cluster biogenesis, essential for mitochondrial function and cellular metabolism. Fe-S clusters are cofactors critical for enzymes involved in DNA repair, electron transport, and redox regulation. NARFL interacts with the CIA (Cytosolic Iron-sulfur cluster Assembly) machinery, facilitating Fe-S cluster transfer to cytosolic and nuclear proteins. Dysregulation of NARFL is linked to mitochondrial disorders, anemia, and cancers due to its role in maintaining genomic stability and energy production.
NARFL antibodies are vital tools for studying Fe-S cluster-related pathways, enabling researchers to detect protein expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies have advanced investigations into diseases like sideroblastic anemia, neurodegenerative conditions (e.g., Friedreich’s ataxia), and tumorigenesis, where Fe-S cluster defects are implicated. Recent studies also explore NARFL's potential as a biomarker or therapeutic target. By elucidating NARFL's molecular mechanisms, these antibodies contribute to understanding cellular iron homeostasis and developing treatments for metabolic and mitochondrial pathologies.
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