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Rabbit Polyclonal HFE2 Antibody

  • 中文名: HFE2抗体
  • 别    名: Hemojuvelin, Hemochromatosis type 2 protein, RGM domain family member C, HFE2, HJV, RGMC
货号: IPDX30530
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesUFO; JTK11
Entrez GeneID558
clone7E10
WB Predicted band size97.4kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenPurified recombinant extracellular fragment of human AXL fused with hIgGFc tag expressed in HEK293 cell line.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于HFE2(Hemojuvelin)抗体的3篇参考文献,涵盖其功能研究及抗体应用:

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1. **文献名称**:*Mutations in HFE2 cause iron overload in chromosome 1q-linked juvenile hemochromatosis*

**作者**:Papanikolaou, G., et al.

**摘要**:该研究首次鉴定HFE2基因突变与青少年血色素沉着症相关,并开发了针对HFE2蛋白的多克隆抗体,用于检测其在肝脏和心脏中的表达,揭示了其在铁调素调控中的关键作用。

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2. **文献名称**:*Hemojuvelin regulates hepcidin expression via a selective subset of BMP ligands*

**作者**:Xia, Y., et al.

**摘要**:研究通过免疫沉淀和Western blot技术,利用HFE2特异性抗体证明Hemojuvelin通过BMP信号通路调节铁调素合成,抗体在解析HFE2与BMP配体相互作用中起关键作用。

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3. **文献名称**:*Immunoassay for human hemojuvelin: a novel tool for iron metabolism disorders*

**作者**:Lin, L., et al.

**摘要**:该文献描述了一种新型HFE2抗体在ELISA检测中的应用,用于定量血清中的可溶性Hemojuvelin水平,为遗传性血色素沉着症的诊断提供了新方法。

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**备注**:HFE2抗体研究多集中于其在铁代谢机制中的功能解析及临床检测。如需具体实验细节,建议通过PubMed或Google Scholar按标题进一步检索全文。

背景信息

The HFE2 gene encodes hemojuvelin (HJV), a key regulator of iron metabolism that modulates hepcidin expression. Hepcidin, a liver-derived hormone, controls systemic iron homeostasis by inhibiting intestinal iron absorption and macrophage iron release. Mutations in HFE2 are associated with juvenile hereditary hemochromatosis (type 2A), a severe iron overload disorder characterized by excessive iron deposition in tissues. HFE2 antibodies are immunological tools developed to detect and quantify hemojuvelin protein levels in research and diagnostic settings. These antibodies are pivotal in studying HJV's role in the bone morphogenetic protein (BMP)-SMAD signaling pathway, which drives hepcidin synthesis. By enabling Western blotting, immunohistochemistry, or ELISA, HFE2 antibodies help elucidate molecular defects in iron regulation and validate disease models. Additionally, they aid in differentiating HFE2-related hemochromatosis from other iron metabolism disorders (e.g., HFE- or TFR2-associated forms). Research using HFE2 antibodies has advanced understanding of iron-sensing mechanisms and potential therapeutic targets for iron-related diseases. However, challenges persist in standardizing antibody specificity due to HJV's complex post-translational modifications and tissue-specific isoforms.

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