| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gamma-glutamylcyclotransferase, Cytochrome c-releasing factor 21, GGCT, C7orf24, CRF21 |
| Entrez GeneID | 79017 |
| WB Predicted band size | 21.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GGCT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 139-168 amino acids from the C-terminal region of human GGCT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GGCT抗体的3篇参考文献示例：
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1. **文献名称**：*Gamma-glutamyl cyclotransferase as a novel therapeutic target in cancer*
**作者**：Smith A, et al.
**摘要**：本研究利用特异性GGCT抗体检测多种癌细胞系中的蛋白表达水平，发现GGCT在乳腺癌和肺癌中显著上调。通过抗体介导的免疫组化分析，证实其高表达与患者生存率降低相关，提示其作为癌症治疗靶点的潜力。
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2. **文献名称**：*Development of a monoclonal antibody targeting human GGCT for diagnostic applications*
**作者**：Tanaka K, et al.
**摘要**：研究团队开发了一种高亲和力的GGCT单克隆抗体，验证其在ELISA和免疫印迹中的特异性。该抗体成功用于检测血清中GGCT水平，为癌症早期诊断提供了新型生物标志物检测工具。
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3. **文献名称**：*Structural and functional analysis of GGCT in glutathione metabolism*
**作者**：Wang L, et al.
**摘要**：通过抗体重组表达和免疫共沉淀技术，揭示了GGCT在谷胱甘肽代谢中的关键作用。研究发现，抑制GGCT活性(通过抗体阻断)可增强化疗药物对肝癌细胞的敏感性，为联合治疗策略提供依据。
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(注：以上文献信息为模拟示例，实际引用时请核对真实数据库。)
The GGCT (gamma-glutamyl cyclotransferase) antibody is a tool used to study the enzyme GGCT, which plays a critical role in glutathione metabolism. GGCT catalyzes the conversion of gamma-glutamyl dipeptides into 5-oxoproline and free amino acids, a key step in the γ-glutamyl cycle responsible for glutathione homeostasis. Glutathione, a major cellular antioxidant, is vital for detoxification, redox balance, and apoptosis regulation. Dysregulation of GGCT has been linked to oxidative stress-related pathologies, including neurodegenerative diseases, cancer, and aging.
GGCT antibodies are primarily used in research to detect and quantify GGCT expression in cells and tissues. Studies highlight its overexpression in cancers like glioblastoma, breast, and lung tumors, suggesting its potential as a diagnostic biomarker or therapeutic target. These antibodies enable techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to explore GGCT's localization, expression patterns, and interaction networks.
Most GGCT antibodies are produced using recombinant GGCT protein fragments as immunogens, often in rabbit or mouse hosts. Validation includes specificity tests (knockdown/knockout controls) and cross-reactivity assessments. Recent applications focus on understanding GGCT's role in tumor progression, chemoresistance, and its interplay with pathways like mTOR and Nrf2. Despite progress, challenges remain in standardizing detection protocols and elucidating precise mechanistic roles in disease contexts.
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