| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Regulator of nonsense transcripts 3B, Nonsense mRNA reducing factor 3B, Up-frameshift suppressor 3 homolog B, hUpf3B, Up-frameshift suppressor 3 homolog on chromosome X, hUpf3p-X, UPF3B, RENT3B, UPF3X |
| Entrez GeneID | 65109 |
| WB Predicted band size | 57.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UPF3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-349 amino acids from the Central region of human UPF3B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于UPF3B抗体的3篇参考文献示例(内容为模拟概括，非真实文献)：
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1. **文献名称**：*UPF3B regulates neurodevelopmental disorders by mediating nonsense-mediated mRNA decay*
**作者**：Nguyen LS et al.
**摘要**：该研究揭示了UPF3B基因突变与X连锁智力障碍的相关性。通过使用UPF3B特异性抗体进行蛋白质表达分析，发现患者细胞中UPF3B水平显著降低，导致NMD通路功能受损，异常转录本积累，进而影响神经发育。
2. **文献名称**：*The role of UPF3B in cancer progression and its interaction with tumor suppressor pathways*
**作者**：Serin G et al.
**摘要**：研究利用UPF3B抗体检测多种癌症组织中该蛋白的表达水平，发现其在乳腺癌和结直肠癌中表达下调。实验表明，UPF3B缺失通过阻碍NMD依赖的致癌转录本降解，促进肿瘤侵袭和转移。
3. **文献名称**：*UPF3B-dependent NMD controls synaptic plasticity and memory formation*
**作者**：Kurosaki T et al.
**摘要**：通过在小鼠模型中敲除UPF3B并结合抗体标记技术，研究发现UPF3B缺失导致海马区神经元突触可塑性受损。机制上，UPF3B通过降解突触抑制相关基因的异常mRNA，维持记忆形成通路的稳态。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“UPF3B antibody”或“UPF3B function”，并筛选涉及抗体应用的研究。
The UPF3B antibody targets the UPF3B protein, a critical component of the nonsense-mediated mRNA decay (NMD) pathway. UPF3B, along with UPF2 and UPF1. forms the core NMD machinery responsible for detecting and degrading mRNAs harboring premature termination codons (PTCs), thereby preventing the production of truncated proteins. UPF3B acts as a bridging factor, linking the exon junction complex (EJC) deposited during splicing to the NMD effector proteins. It plays a role in both canonical and alternative NMD pathways, contributing to mRNA quality control and gene expression regulation.
Mutations in the UPF3B gene are linked to X-linked intellectual disability (XLID) and neurodevelopmental disorders, underscoring its importance in neuronal function. Researchers use UPF3B antibodies in applications like Western blotting, immunofluorescence, and immunoprecipitation to study its expression, localization, and interactions. These antibodies are essential for investigating NMD dysregulation in diseases such as cancer, neurodegeneration, and genetic disorders caused by PTCs. Validated UPF3B antibodies typically show specificity for human, mouse, or rat samples, aiding in comparative studies across model systems. Its dual role in RNA surveillance and developmental regulation makes UPF3B a protein of broad interest in molecular biology and translational research.
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