| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | F-box only protein 4, FBXO4, FBX4 |
| Entrez GeneID | 26272 |
| WB Predicted band size | 44.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FBXO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-251 amino acids from the Central region of human FBXO4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FBXO4抗体的参考文献示例(注：以下内容为模拟虚构，仅供参考格式)：
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1. **文献名称**: "FBXO4-mediated degradation of Cyclin D1 regulates cellular proliferation"
**作者**: Lin DI, et al.
**摘要**: 该研究揭示了FBXO4通过SCF泛素连接酶复合体调控Cyclin D1的泛素化降解，抑制细胞周期进程。实验中采用FBXO4特异性抗体进行Western blot和免疫共沉淀，验证了FBXO4与Cyclin D1的相互作用及功能关联。
2. **文献名称**: "Loss of FBXO4 expression correlates with poor prognosis in colorectal cancer"
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化(使用FBXO4抗体)分析结直肠癌组织样本，发现FBXO4低表达与肿瘤分期及患者生存率显著相关，提示其作为潜在预后标志物的可能性。
3. **文献名称**: "Phosphorylation-dependent regulation of FBXO4 in DNA damage response"
**作者**: Chen L, et al.
**摘要**: 研究阐明了FBXO4在DNA损伤修复中的功能，利用FBXO4抗体检测其磷酸化修饰状态，证明ATM激酶介导的磷酸化调控FBXO4与底物的结合能力。
4. **文献名称**: "FBXO4 mutations disrupt substrate recognition in breast cancer"
**作者**: Gupta R, et al.
**摘要**: 在乳腺癌患者中发现FBXO4基因突变，通过免疫沉淀(使用FBXO4抗体)和质谱分析，揭示突变体丧失对特定底物的识别能力，导致致癌蛋白积累。
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**注意**：以上文献为示例，实际引用时请通过PubMed、Web of Science等数据库检索真实存在的文献并核对信息。
The FBXO4 antibody is a crucial tool for studying the F-box protein 4 (FBXO4), a component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex. FBXO4 belongs to the F-box protein family, characterized by an F-box domain that mediates interactions with SKP1. enabling substrate recognition for ubiquitination and subsequent proteasomal degradation. FBXO4 plays a pivotal role in regulating cell cycle progression, stress response, and tumor suppression by targeting specific substrates, such as Cyclin D1. for degradation. Dysregulation of FBXO4 has been linked to cancers, including breast, liver, and lung cancers, where its loss or mutation contributes to uncontrolled cell proliferation and genomic instability.
The FBXO4 antibody is widely used in research to detect FBXO4 expression levels, localization, and interactions in cells or tissues. It aids in elucidating FBXO4's functional mechanisms in physiological and pathological contexts, such as its role in the ubiquitin-proteasome system and tumorigenesis. Applications include Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Commercially available antibodies are typically raised against specific epitopes of human FBXO4. with validation in diverse experimental models. Understanding FBXO4's regulatory pathways through such antibodies holds promise for developing therapeutic strategies targeting SCF complexes or associated oncoproteins in cancer treatment.
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