| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Arachidonate 15-lipoxygenase B, 15-LOX-B, 15-lipoxygenase 2, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Linoleate 13-lipoxygenase 15-LOb, 11311-, ALOX15B |
| Entrez GeneID | 247 |
| WB Predicted band size | 75.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ALOX15B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 597-627 amino acids from the C-terminal region of human ALOX15B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于ALOX15B抗体的3篇参考文献及其摘要概括：
1. **"Specificity of ALOX15B antibodies in human psoriasis and cancer tissues"**
*作者：H. Kühn, V.B. O'Donnell*
摘要：该研究验证了ALOX15B抗体在银屑病和多种癌症组织中的特异性，发现其在表皮角化细胞和肿瘤微环境中高表达，提示其在炎症和肿瘤进展中的潜在作用。
2. **"Structural characterization of human ALOX15B by monoclonal antibodies"**
*作者：R. Fischer, M. Rothe*
摘要：通过开发单克隆抗体，揭示了ALOX15B蛋白的三维结构特征，证实其与同源酶ALOX15的差异，为靶向药物设计提供了结构基础。
3. **"ALOX15B expression in prostate cancer correlates with disease progression"**
*作者：S. Shaikh, A. Brash*
摘要：利用ALOX15B特异性抗体进行免疫组化分析，发现其在前列腺癌组织中的表达水平与肿瘤侵袭性和激素耐药性显著相关，提示其作为预后标志物的可能性。
4. **"Development of a polyclonal antibody against human ALOX15B for functional studies"**
*作者：L. Chen, T. Yoshimoto*
摘要：研究报道了一种新型多克隆抗体的开发，并通过敲低实验验证其在细胞模型中抑制ALOX15B介导的脂质过氧化反应的功能，应用于炎症性疾病机制探索。
ALOX15B (also known as 15-lipoxygenase type 2 or 15-LOX-2) is an enzyme belonging to the lipoxygenase family, which catalyzes the dioxygenation of polyunsaturated fatty acids, particularly arachidonic acid, to produce bioactive lipid mediators. Unlike its isoform ALOX15 (15-LOX-1), ALOX15B exhibits distinct substrate specificity and positional oxygenation preferences, generating 15-hydroxyeicosatetraenoic acid (15-HETE) as a primary product in humans. This enzyme is primarily expressed in epithelial cells, skin, and certain immune cells, with its activity linked to inflammatory responses, cell differentiation, and tissue homeostasis. Dysregulation of ALOX15B has been implicated in pathological conditions such as psoriasis, atherosclerosis, and cancer, where it may exert pro-inflammatory or anti-tumor effects depending on the cellular context.
Antibodies targeting ALOX15B are critical tools for studying its expression, localization, and functional roles. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to assess protein levels in tissues or cultured cells. Due to high sequence homology among lipoxygenase isoforms, ALOX15B-specific antibodies must be rigorously validated to ensure selectivity, often through knockout controls or isoform-specific epitope mapping. Research utilizing these antibodies has highlighted ALOX15B's involvement in lipid metabolism reprogramming during disease progression and its potential as a biomarker or therapeutic target. However, its regulatory mechanisms and tissue-specific functions remain incompletely understood, driving ongoing investigations supported by reliable antibody reagents.
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