| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FAD synthase, FAD pyrophosphorylase, FMN adenylyltransferase, Flavin adenine dinucleotide synthase, Molybdenum cofactor biosynthesis protein-like region, FAD synthase region, FLAD1 |
| Entrez GeneID | 80308 |
| WB Predicted band size | 65.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FLAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 551-580 amino acids from the C-terminal region of human FLAD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FLAD1抗体的3篇示例文献(内容为模拟概括，仅供参考)：
1. **标题**：*FLAD1 mutations cause a novel syndrome of myopathy and intellectual disability by disrupting FAD metabolism*
**作者**：Smith J, et al.
**摘要**：本研究通过全外显子测序发现FLAD1基因突变导致FAD合成酶功能缺陷，引发肌肉病变和神经发育异常。利用特异性FLAD1抗体进行Western blot和免疫荧光，证实患者细胞中FLAD1蛋白表达显著降低。
2. **标题**：*FLAD1 antibody-based profiling reveals its role in mitochondrial respiration and cancer cell proliferation*
**作者**：Chen L, et al.
**摘要**：通过FLAD1抗体检测多种癌细胞系，发现FLAD1高表达与线粒体氧化磷酸化活性增强相关。敲低FLAD1后，细胞增殖受抑制，提示其作为癌症治疗靶点的潜力。
3. **标题**：*Structural characterization of human FLAD1 and its interaction with riboflavin kinase*
**作者**：Gomez-Rodriguez M, et al.
**摘要**：利用FLAD1抗体进行免疫共沉淀(Co-IP)和质谱分析，揭示了FLAD1与核黄素激酶的相互作用机制，阐明其在FAD合成通路中的结构功能。
**备注**：以上文献信息为示例，实际文献需通过PubMed或SciFinder等数据库检索确认。
FLAD1 (Flavin Adenine Dinucleotide Synthetase 1) is a critical enzyme responsible for the biosynthesis of flavin adenine dinucleotide (FAD), an essential cofactor involved in numerous redox reactions and metabolic pathways, including the tricarboxylic acid (TCA) cycle, fatty acid oxidation, and mitochondrial electron transport chain. Encoded by the FLAD1 gene, this enzyme catalyzes the conversion of riboflavin (vitamin B2) into FAD via a two-step ATP-dependent process. FAD serves as a prosthetic group for various flavoproteins, playing a vital role in cellular energy production, antioxidant defense, and DNA repair mechanisms.
Antibodies targeting FLAD1 are valuable tools for studying its expression, localization, and function in both physiological and pathological contexts. Researchers utilize FLAD1 antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate FAD synthesis deficiencies linked to metabolic disorders, mitochondrial dysfunction, and rare genetic conditions such as lipid storage myopathy or multiple acyl-CoA dehydrogenase deficiency (MADD). Additionally, these antibodies aid in exploring FLAD1’s potential involvement in cancer progression, as altered FAD levels may influence redox homeostasis and tumor metabolism. Understanding FLAD1 regulation and activity through antibody-based assays contributes to therapeutic strategies targeting FAD-dependent pathways in metabolic and degenerative diseases.
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