| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bromo adjacent homology domain-containing 1 protein, BAH domain-containing protein 1, BAHD1, KIAA0945 |
| Entrez GeneID | 22893 |
| WB Predicted band size | 84.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 750-777 amino acids from the C-terminal region of human BAHD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BAHD1抗体的3篇参考文献及其简要摘要内容：
1. **文献名称**：*BAHD1 is a nuclear scaffold protein that interacts with heterochromatin and regulates gene silencing*
**作者**：Bierne, H., Tham, T.N., et al.
**摘要**：该研究揭示了BAHD1作为核支架蛋白的功能，通过免疫沉淀(IP)和染色质免疫共沉淀(ChIP)实验，使用BAHD1抗体证实其与异染色质区域结合，并参与基因沉默过程，尤其在细胞分化中调控表观遗传修饰。
2. **文献名称**：*Characterization of BAHD1. a novel protein associated with the bromodomain of polybromo-1*
**作者**：Lemercier, C., Duncliffe, K., et al.
**摘要**：本研究首次报道了BAHD1的分子特征，通过Western blot和免疫荧光技术，利用特异性BAHD1抗体验证其在细胞核内的定位，并发现其与Polycomb复合体相关蛋白的潜在相互作用，提示其在染色质重塑中的作用。
3. **文献名称**：*BAHD1 bridges the LSD1/CoRST complex to the chromatin-binding protein CECR2*
**作者**：Riclet, R., Chendeb, M., et al.
**摘要**：通过免疫共沉淀(Co-IP)和蛋白质互作分析，结合BAHD1抗体的应用，研究发现BAHD1作为桥梁分子连接LSD1/CoRST复合体与染色质结合蛋白CECR2.调控靶基因的转录抑制和染色质结构动态。
**备注**：BAHD1抗体在上述研究中多用于检测蛋白表达、定位及相互作用分析，相关文献可进一步通过PubMed或Google Scholar以关键词“BAHD1 antibody”或“BAHD1 chromatin interaction”检索。
The BAHD1 (Bromodomain Adjacent to Histone Domain 1) antibody is a research tool designed to detect and study the BAHD1 protein, a chromatin-associated factor involved in epigenetic regulation. BAHD1 is implicated in heterochromatin formation, transcriptional repression, and genomic stability through interactions with histone-modifying complexes (e.g., Polycomb repressors) and DNA methyltransferases. It contains a bromodomain, which typically binds acetylated lysine residues on histones, and a conserved HAHD (Histone Associated Heterochromatin Domain) thought to mediate chromatin interactions. BAHD1 has been linked to cellular processes like differentiation, apoptosis, and viral latency, as well as diseases including cancer and metabolic disorders.
BAHD1 antibodies, often developed in rabbits or mice as polyclonal or monoclonal versions, are validated for applications like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity is confirmed using knockout controls or siRNA-mediated knockdown. These antibodies enable researchers to investigate BAHD1's role in silencing specific gene loci, its colocalization with repressive histone marks (e.g., H3K27me3. H3K9me3), and its interplay with pathways like TGF-β signaling. Challenges include low endogenous protein levels in some cell types, necessitating sensitive detection methods. BAHD1 antibodies remain critical for exploring epigenetic mechanisms and potential therapeutic targets.
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