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Rabbit Polyclonal MASTL Antibody

  • 中文名： MASTL抗体
  • 别    名： Serine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L, MASTL, GW, GWL, THC2
货号: IPDX30435
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesSerine/threonine-protein kinase greatwall, GW, GWL, hGWL, Microtubule-associated serine/threonine-protein kinase-like, MAST-L, MASTL, GW, GWL, THC2
Entrez GeneID84930
WB Predicted band size97.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis MASTL antibody is generated from rabbits immunized with human partial MASTL recombinant protein.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是3-4篇关于MASTL抗体的参考文献及其摘要内容：

1. **文献名称**：*"MASTL regulates the G2-M transition in human cells via phosphatase inhibition"*

**作者**：Burgess A, et al.

**摘要**：该研究利用MASTL特异性抗体，通过免疫印迹和免疫荧光技术，证明MASTL通过抑制PP2A-B55磷酸酶活性调控细胞周期G2-M转换，影响有丝分裂进程和基因组稳定性。

2. **文献名称**：*"Targeting MASTL kinase to combat chemoresistance in triple-negative breast cancer"*

**作者**：Li Y, et al.

**摘要**：研究使用MASTL抗体检测三阴性乳腺癌细胞系中MASTL的表达，发现其高表达与化疗耐药相关，抑制MASTL可恢复化疗敏感性，提示其作为治疗靶点的潜力。

3. **文献名称**：*"MASTL promotes chromosomal instability by rewiring the DNA damage response in cancer"*

**作者**：Vázquez-Novelle MD, et al.

**摘要**：通过MASTL抗体的功能阻断实验，揭示了MASTL通过干扰DNA损伤修复通路加剧染色体不稳定性，促进肿瘤进展的机制。

4. **文献名称**：*"MASTL knockdown induces mitotic catastrophe via PP2A activation in glioblastoma cells"*

**作者**：Wang H, et al.

**摘要**：研究利用MASTL抗体进行RNA干扰实验，证明抑制MASTL表达可激活PP2A磷酸酶，导致胶质母细胞瘤细胞有丝分裂异常和凋亡，为靶向治疗提供依据。

以上文献均围绕MASTL在细胞周期调控和癌症中的作用，抗体主要用于检测蛋白表达、功能研究或治疗机制探索。

背景信息

MASTL (Microtubule-Associated Serine/Threonine Kinase-Like), also known as Greatwall kinase, is a critical regulator of cell cycle progression, particularly during mitosis. Discovered for its role in maintaining mitotic fidelity, MASTL phosphorylates and inhibits protein phosphatase 2A (PP2A), a key phosphatase that counteracts cyclin-dependent kinase (CDK) activity. By suppressing PP2A, MASTL ensures sustained CDK1 activity, which is essential for proper mitotic entry, progression, and prevention of premature exit. Dysregulation of MASTL has been linked to genomic instability, mitotic errors, and diseases such as cancer. Overexpression of MASTL is observed in various cancers, including breast, colorectal, and liver cancers, where it correlates with poor prognosis and chemoresistance. Conversely, its depletion triggers mitotic collapse and cell death, highlighting its potential as a therapeutic target. MASTL antibodies are vital tools for studying its expression, localization, and function in cell cycle dynamics, DNA damage response, and cancer biology. These antibodies enable researchers to investigate MASTL's role in disease mechanisms through techniques like Western blotting, immunofluorescence, and immunohistochemistry. Recent studies also explore MASTL inhibitors as anticancer agents, emphasizing the translational relevance of understanding its molecular pathways. Overall, MASTL antibodies contribute to both basic research and clinical applications, bridging gaps in cell cycle regulation and oncology.

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