| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12, CASP2, ICH1, NEDD2 |
| Entrez GeneID | 835 |
| WB Predicted band size | 50.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CASP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the Central region of human CASP2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是关于CASP2(Caspase-2)抗体的3篇参考文献及其摘要概括：
1. **文献名称**：*Caspase-2: controversial killer or checkpoint controller?*
**作者**：Kumar S.
**摘要**：该综述探讨了Caspase-2在细胞凋亡和维持基因组稳定性中的双重作用，提到通过特异性抗体(如CASP2抗体)检测其在细胞核和胞质中的定位变化，支持其在DNA损伤后激活下游凋亡通路的假说。
2. **文献名称**：*Caspase-2 is required for DNA damage-induced apoptosis following mitotic catastrophe*
**作者**：Vakifahmetoglu-Norberg H, et al.
**摘要**：研究利用CASP2抗体通过Western blot和免疫荧光技术，证明Caspase-2在DNA损伤诱导的细胞凋亡中不可或缺，尤其在“有丝分裂灾难”后通过激活线粒体凋亡途径清除异常细胞。
3. **文献名称**：*Caspase-2 cleavage of tau reversibly impairs synaptic function*
**作者**：Uribe V, et al.
**摘要**：文章通过CASP2抗体结合免疫沉淀技术，发现Caspase-2在阿尔茨海默病模型中特异性切割tau蛋白，导致突触功能障碍，提示其作为神经退行性疾病的潜在治疗靶点。
(注：以上文献为示例性概括，实际引用需核对原文信息及数据库。)
**Background of CASP2 Antibodies**
Caspase-2 (CASP2), a member of the cysteine-aspartic protease family, plays multifaceted roles in apoptosis, genome stability, and tumor suppression. Structurally, it contains a caspase recruitment domain (CARD) and a catalytic protease domain, existing as an inactive zymogen until proteolytic activation. CASP2 is unique for its dual activation pathways: intrinsic (via PIDDosome complex) and extrinsic (through DISC assembly), enabling context-dependent responses to DNA damage, metabolic stress, or developmental cues.
CASP2 antibodies are critical tools for studying its expression, localization, and function. They detect full-length (∼48 kDa) and cleaved active forms (∼34 kDa and ∼14 kDa subunits) in immunoblotting (WB), immunohistochemistry (IHC), or immunofluorescence (IF). These antibodies aid in exploring CASP2's role in cleaving substrates (e.g., BID, Golgin-160) to trigger apoptosis or regulate cellular homeostasis.
Research using CASP2 antibodies has linked its dysregulation to cancer, neurodegenerative diseases, and developmental disorders. For instance, reduced CASP2 correlates with chemoresistance, while hyperactivation may drive neuronal death. However, challenges persist due to overlapping substrates with other caspases and complex post-translational modifications (e.g., phosphorylation). Validated, isoform-specific antibodies are essential to dissect CASP2's context-dependent mechanisms and therapeutic potential.
×
