| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeodomain-interacting protein kinase 2, hHIPk2, HIPK2 |
| Entrez GeneID | 28996 |
| WB Predicted band size | 131.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This HIPK2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y361 of human HIPK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-HIPK2(Y361)抗体的**模拟参考文献示例**(请注意，以下内容为假设性示例，实际文献需通过学术数据库核实)：
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1. **文献名称**: *HIPK2 Phosphorylation at Tyrosine 361 Modulates p53-Mediated Apoptosis in DNA Damage Response*
**作者**: Smith J, et al.
**摘要**: 研究揭示了HIPK2在Y361位点的磷酸化通过增强其激酶活性，促进p53蛋白的稳定性，从而调控DNA损伤诱导的细胞凋亡。该抗体被用于验证Y361磷酸化在肿瘤细胞中的功能。
2. **文献名称**: *Phospho-HIPK2(Y361) as a Novel Biomarker in Wnt/β-Catenin Signaling Activation*
**作者**: Lee H, et al.
**摘要**: 发现Y361磷酸化的HIPK2通过促进β-catenin的核转位激活Wnt信号通路，与结直肠癌进展相关。研究使用该抗体进行组织芯片分析，证实其临床相关性。
3. **文献名称**: *Oxidative Stress Induces HIPK2 Tyrosine 361 Phosphorylation via c-Abl Kinase*
**作者**: Rossi M, et al.
**摘要**: 阐明氧化应激通过c-Abl激酶介导HIPK2 Y361位点磷酸化，进而调控下游JNK/p38通路，影响神经元存活。该抗体用于体外磷酸化水平检测。
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**建议**：
如需真实文献，可通过以下途径检索：
- **PubMed/Google Scholar**：关键词“HIPK2 Y361 phosphorylation”或“Phospho-HIPK2(Y361) antibody”。
- **抗体公司数据库**：如CST、Abcam等厂商可能提供相关抗体的引用文献列表。
- **领域综述**：查找HIPK2在凋亡、癌症或神经退行性疾病中的综述，追踪原始研究。
希望以上示例对您的研究方向有所启发!
Phospho-HIPK2(Y361) antibody is a specialized tool used to detect the phosphorylated form of Homeodomain-interacting protein kinase 2 (HIPK2) at tyrosine residue 361. HIPK2. a serine/threonine kinase, plays critical roles in cellular stress responses, DNA damage signaling, apoptosis, and regulation of transcriptional pathways. Its activity is tightly controlled through post-translational modifications, including phosphorylation. The phosphorylation of tyrosine 361 (Y361) is associated with HIPK2 activation, often triggered by stressors like UV radiation, oxidative stress, or chemotherapeutic agents. This modification enhances HIPK2’s kinase activity and promotes its nuclear localization, enabling interactions with transcription factors such as p53. Wnt/β-catenin, and Smad proteins to modulate gene expression.
The Phospho-HIPK2(Y361) antibody is widely utilized in research to investigate HIPK2’s regulatory mechanisms in diseases like cancer, neurodegenerative disorders, and fibrotic conditions. For example, dysregulated HIPK2 phosphorylation has been linked to tumor progression, where it may either promote apoptosis or survival depending on cellular context. This antibody is validated for applications like Western blotting, immunofluorescence, and immunohistochemistry, often in models ranging from cell lines to tissue samples. Researchers rely on its specificity to explore HIPK2 signaling dynamics, offering insights into therapeutic targets for diseases driven by stress-response pathway imbalances. Proper controls, such as phosphorylation-blocking peptides or phosphatase treatment, are essential to confirm signal specificity.
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