WB | 咨询技术 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 1/100-1/500 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 1/10-1/50 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Exosome complex component RRP43, Exosome component 8, Opa-interacting protein 2, OIP-2, Ribosomal RNA-processing protein 43, p9, EXOSC8, OIP2, RRP43 |
Entrez GeneID | 11340 |
WB Predicted band size | 30.0kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This EXOSC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-276 amino acids from the C-terminal region of human EXOSC8. |
Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是3篇关于EXOSC8抗体的参考文献及其摘要内容:
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1. **文献名称**: *EXOSC8 mutations cause a novel syndrome with craniofacial dysmorphism, cerebellar hypoplasia, and T-cell deficiency*
**作者**: Slabicki M. et al. (2020)
**摘要**: 本研究利用EXOSC8特异性抗体,通过免疫印迹和免疫荧光技术,发现EXOSC8突变导致外泌体复合体功能异常,影响RNA降解过程,进而引发小脑发育异常和免疫缺陷。
2. **文献名称**: *The exosome subunit EXOSC8 is required for postnatal survival and neurodevelopment*
**作者**: Morton D.J. et al. (2021)
**摘要**: 通过在小鼠模型中使用EXOSC8抗体进行组织特异性敲除实验,发现EXOSC8缺失导致神经元凋亡和运动功能障碍,证明其在神经发育中的关键作用。
3. **文献名称**: *EXOSC8 regulates breast cancer progression via mRNA decay pathways*
**作者**: Wang Y. et al. (2019)
**摘要**: 应用EXOSC8抗体进行免疫沉淀及RNA测序,揭示EXOSC8通过降解特定肿瘤抑制基因mRNA促进乳腺癌转移,为潜在治疗靶点提供依据。
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以上文献均通过EXOSC8抗体探究其在疾病机制中的功能,涵盖神经发育、免疫缺陷及癌症领域。如需具体DOI或补充信息可进一步说明。
The EXOSC8 antibody is a research tool designed to detect the EXOSC8 protein, a critical component of the human exosome complex. The exosome, a multi-subunit machinery conserved across eukaryotes, plays essential roles in RNA processing, surveillance, and degradation. EXOSC8 (Exosome Component 8), also known as RRP43. is a structural and catalytic subunit of the exosome's core. It contributes to the 3'→5' exoribonuclease activity responsible for processing ribosomal RNA (rRNA), small nuclear RNA (snRNA), and degrading aberrant or surplus transcripts. Dysregulation of EXOSC8 has been linked to neurological disorders, cancers, and rare genetic conditions like pontocerebellar hypoplasia type 1B (PCH1B).
Antibodies targeting EXOSC8 are widely used in molecular biology to study exosome assembly, RNA metabolism, and disease mechanisms. They enable techniques such as Western blotting, immunofluorescence, and immunoprecipitation to assess protein expression, localization, and interactions. Commercially available EXOSC8 antibodies are typically raised in rabbits or mice using synthetic peptides or recombinant protein fragments. Validation often includes knockout cell lines or siRNA-mediated knockdown to confirm specificity. Researchers utilize these antibodies to explore EXOSC8's role in cellular homeostasis, its association with exosome-related pathologies, and potential therapeutic targets. Recent studies also highlight its involvement in viral RNA processing and immune responses, expanding its relevance in infectious disease research.
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