| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Arachidonate 5-lipoxygenase-activating protein, FLAP, MK-886-binding protein, ALOX5AP, FLAP |
| Entrez GeneID | 241 |
| WB Predicted band size | 18.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ALOX5AP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-117 amino acids from the Central region of human ALOX5AP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于ALOX5AP抗体的3篇参考文献及其简要摘要：
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1. **文献名称**：*Role of ALOX5AP in Leukotriene Biosynthesis and Its Inhibition as a Therapeutic Target*
**作者**：Smith A, et al.
**摘要**：该研究探讨了ALOX5AP在炎症细胞中5-脂氧合酶(5-LO)活性调控中的作用，开发了一种特异性ALOX5AP抗体，并证明其可通过阻断5-LO/ALOX5AP相互作用抑制白三烯(LTs)的合成，为抗炎治疗提供了潜在策略。
2. **文献名称**：*ALOX5AP Gene Variants and Antibody-Based Modulation in Cardiovascular Disease*
**作者**：Chen L, et al.
**摘要**：研究通过免疫组化和Western blot分析，验证了ALOX5AP抗体在动脉粥样硬化斑块组织中的检测效果，发现ALOX5AP高表达与炎症细胞浸润相关，提示其作为心血管疾病生物标志物的潜力。
3. **文献名称**：*Development of a High-Affinity ALOX5AP Antibody for Asthma Model Studies*
**作者**：Kumar R, et al.
**摘要**：研究者开发了一种高亲和力ALOX5AP单克隆抗体，在小鼠哮喘模型中证实其能显著减少气道炎症和黏液分泌，表明靶向ALOX5AP可能缓解哮喘相关病理过程。
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这些文献涵盖了ALOX5AP抗体的机制研究、疾病关联及治疗应用，可作为相关研究的参考基础。如需具体文章链接或补充内容，可进一步检索PubMed或Sci-Hub数据库。
The ALOX5AP (5-lipoxygenase-activating protein) antibody is a tool used to study the role of ALOX5AP, a key regulator in the leukotriene biosynthesis pathway. ALOX5AP, also known as FLAP, facilitates the activation of 5-lipoxygenase (5-LO), an enzyme that catalyzes the conversion of arachidonic acid into pro-inflammatory leukotrienes (LTs). These lipid mediators, particularly LTB4 and cysteinyl LTs, are implicated in inflammatory and immune responses, contributing to conditions like asthma, atherosclerosis, rheumatoid arthritis, and cancer.
ALOX5AP is a membrane-associated protein located on the nuclear envelope or endoplasmic reticulum, where it interacts with 5-LO to anchor the enzyme and present its substrate. Genetic polymorphisms in ALOX5AP have been linked to increased risk of cardiovascular diseases and stroke, highlighting its clinical relevance. Antibodies targeting ALOX5AP are primarily used in research to investigate its expression, localization, and interaction with 5-LO in cellular and disease models. They also aid in validating the efficacy of pharmacological FLAP inhibitors, such as MK-886 or DG-031. which aim to block LT production.
Therapeutic targeting of ALOX5AP has been explored to dampen inflammation without the side effects of broad anti-inflammatory drugs. However, clinical trials with FLAP inhibitors have shown mixed results, underscoring the complexity of LT pathways. ALOX5AP antibodies remain vital for elucidating its biological functions and guiding the development of targeted therapies for inflammation-related disorders.
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