| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nicotinamide riboside kinase 2, NRK 2, NmR-K 2, Integrin beta-1-binding protein 3, Muscle integrin-binding protein, MIBP, Nicotinic acid riboside kinase 2, Ribosylnicotinamide kinase 2, RNK 2, Ribosylnicotinic acid kinase 2, NMRK2, ITGB1BP3, NRK2 |
| Entrez GeneID | 27231 |
| WB Predicted band size | 26.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ITGB1BP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human ITGB1BP3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是几篇关于ITGB1BP3(N-term)抗体的参考文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询确认)：
1. **文献名称**：*ITGB1BP3 regulates integrin β1-mediated cell adhesion via N-terminal interactions*
**作者**：Smith A, et al.
**摘要**：本研究揭示了ITGB1BP3通过其N端结构域与整合素β1的相互作用，调控细胞黏附和迁移的分子机制。实验中采用ITGB1BP3(N-term)抗体进行免疫共沉淀和Western blot分析，证实了其在信号通路中的关键作用。
2. **文献名称**：*Expression profiling of ITGB1BP3 in tumor microenvironment using N-terminal specific antibody*
**作者**：Li X, et al.
**摘要**：通过ITGB1BP3(N-term)抗体的免疫组化染色，研究发现ITGB1BP3在多种肿瘤微环境中高表达，并与血管生成相关。该抗体特异性识别N端表位，为肿瘤靶向治疗提供了潜在标志物。
3. **文献名称**：*Functional characterization of ITGB1BP3 in extracellular matrix remodeling*
**作者**：Wang Y, et al.
**摘要**：利用ITGB1BP3(N-term)抗体进行免疫荧光和流式细胞术，证明ITGB1BP3通过调控整合素β1的活性参与细胞外基质重塑，影响纤维母细胞的迁移和侵袭能力。
4. **文献名称**：*ITGB1BP3 N-terminal antibody validation for kinase signaling studies*
**作者**：Garcia R, et al.
**摘要**：本文系统验证了ITGB1BP3(N-term)抗体的特异性，并应用于磷酸化蛋白检测，发现ITGB1BP3的N端结构域参与MAPK信号通路的调控。
**建议**：具体文献请通过PubMed、Google Scholar或抗体供应商(如Abcam、Cell Signaling Technology)提供的产品引用页面查询。
The ITGB1BP3 (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of Integrin Beta-1 Binding Protein 3 (ITGB1BP3), also known as MIBP3 or C9orf14. ITGB1BP3 is a cytoplasmic protein that interacts with the integrin β1 subunit, playing a role in regulating integrin-mediated signaling pathways involved in cell adhesion, migration, and extracellular matrix interactions. It is implicated in modulating cellular responses to mechanical stimuli and has been studied in contexts such as cancer progression, fibrosis, and vascular remodeling.
This antibody is commonly used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect endogenous ITGB1BP3 protein levels in various biological samples. The N-terminal specificity ensures recognition of full-length or truncated forms of the protein, aiding in studies of its expression, localization, and post-translational modifications. Researchers often validate the antibody using knockout cell lines or siRNA-mediated knockdown to confirm target specificity. ITGB1BP3’s involvement in integrin signaling pathways, particularly those linked to FAK (focal adhesion kinase) and MAPK cascades, makes this antibody a valuable tool for investigating cell-matrix interactions and mechanotransduction mechanisms. Optimal dilution ratios and experimental conditions may vary depending on sample type and assay requirements.
×
