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Rabbit Polyclonal Phospho-MeCP2(S80) Antibody

  • 中文名： Phospho-MeCP2(S80)抗体
  • 别    名： Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
货号: IPDX30323
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesMethyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Entrez GeneID4204
WB Predicted band size52.4kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S80 of human MeCP2.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-MeCP2(S80)抗体的虚构参考文献示例，基于相关领域的研究方向整理：

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1. **文献名称**：*Dynamic Phosphorylation of MeCP2 at Serine 80 Regulates Synaptic Plasticity*

**作者**：Tao, J., et al. (2009)

**摘要**：本研究利用Phospho-MeCP2(S80)特异性抗体，揭示了MeCP2在神经元活动中的磷酸化动态变化。通过免疫印迹和免疫荧光技术，发现S80位点的磷酸化响应于NMDA受体激活，并调控MeCP2与染色质重塑复合物的相互作用，影响突触可塑性和学习记忆功能。

2. **文献名称**：*MeCP2 Phosphorylation at Ser80 Modulates DNA Binding and Gene Repression*

**作者**：Guy, J., et al. (2011)

**摘要**：通过Phospho-MeCP2(S80)抗体，作者发现S80磷酸化会减弱MeCP2与甲基化DNA的结合能力，并影响其招募组蛋白去乙酰化酶(HDAC)的能力。该修饰在神经元分化过程中动态变化，提示其在神经发育中的关键作用。

3. **文献名称**：*Aberrant MeCP2(S80) Phosphorylation in Rett Syndrome Models*

**作者**：Li, W., et al. (2013)

**摘要**：本研究使用Phospho-MeCP2(S80)抗体分析Rett综合征小鼠模型，发现S80磷酸化水平显著降低。这种异常导致MeCP2靶基因(如BDNF)的转录失调，为疾病机制提供了新的分子解释。

4. **文献名称**：*Development and Validation of a Phospho-Specific MeCP2(S80) Antibody for Epigenetic Studies*

**作者**：Ebert, D.H., et al. (2014)

**摘要**：文章详细描述了Phospho-MeCP2(S80)抗体的制备与验证，包括肽段免疫、特异性检测(如磷酸化位点突变体对照)及在脑组织中的应用。该抗体为研究MeCP2翻译后修饰提供了可靠工具。

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*注：以上文献为示例性质，实际引用时需核实真实来源。建议通过PubMed或Google Scholar以关键词“MeCP2 S80 phosphorylation”或抗体货号(如厂商提供的参考文献)查找真实文献。*

背景信息

The Phospho-MeCP2(S80) antibody is a specialized tool used to study post-translational modifications of methyl-CpG-binding protein 2 (MeCP2), a chromatin-associated protein critical for neurodevelopment. MeCP2 regulates gene expression by binding methylated DNA and recruiting transcriptional repressors, and its dysfunction is linked to Rett syndrome and other neurological disorders. Phosphorylation at serine 80 (S80) is a key regulatory mechanism modulating MeCP2’s interactions with chromatin and its ability to influence synaptic plasticity. This site-specific phosphorylation, often triggered by neuronal activity or calcium-dependent signaling (e.g., via CaMKII), fine-tunes MeCP2’s role in activity-dependent gene repression or activation. The Phospho-MeCP2(S80) antibody enables precise detection of this modification in techniques like Western blotting, immunofluorescence, or immunohistochemistry, helping researchers explore dynamic MeCP2 regulation in cellular or animal models. Studies using this antibody have revealed how S80 phosphorylation impacts MeCP2’s dissociation from DNA, its interaction with co-repressors (e.g., HDACs/Sin3a), and its role in neurological disease mechanisms. Its application has advanced understanding of epigenomic dysregulation in disorders like Rett syndrome, autism, and epilepsy, providing insights into therapeutic strategies targeting MeCP2 modifications.

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