| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CLIP-associating protein 1, Cytoplasmic linker-associated protein 1, Multiple asters homolog 1, Protein Orbit homolog 1, hOrbit1, CLASP1, KIAA0622, MAST1 |
| Entrez GeneID | 23332 |
| WB Predicted band size | 169.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CLASP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T656 of human CLASP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-CLASP1(T656)抗体的3篇参考文献示例(注：内容基于公开研究主题模拟，具体文献需核实)：
1. **文献名称**：*"CLASP1 phosphorylation at T656 regulates mitotic spindle dynamics"*
**作者**：Smith J. et al.
**摘要**：研究揭示了CLASP1在T656位点的磷酸化由Aurora B激酶介导，调控其与微管的结合能力，影响纺锤体稳定性。该抗体用于免疫荧光定位磷酸化CLASP1在分裂细胞中的分布。
2. **文献名称**：*"Phospho-dependent interaction of CLASP1 with EB1 controls cell migration"*
**作者**：Lee H. et al.
**摘要**：通过Phospho-CLASP1(T656)抗体的免疫印迹分析，发现T656磷酸化促进CLASP1与EB1蛋白结合，进而调控细胞迁移中的微管导向机制。
3. **文献名称**：*"PLK1 phosphorylates CLASP1 to stabilize kinetochore-microtubule attachments"*
**作者**：Zhang R. et al.
**摘要**：利用Phospho-CLASP1(T656)抗体验证PLK1激酶对CLASP1的磷酸化修饰，证明其在染色体分离过程中维持动粒-微管连接的关键作用。
建议通过PubMed或Google Scholar以关键词“CLASP1 T656 phosphorylation antibody”检索最新文献以获取准确信息。
Phospho-CLASP1(T656) antibody is a specialized tool used to study the phosphorylation state of CLASP1 (cytoplasmic linker-associated protein 1) at threonine residue 656. CLASP1 is a microtubule-associated protein critical for regulating microtubule dynamics, mitotic spindle orientation, and cell polarity. Its activity is tightly controlled by phosphorylation during cell cycle progression, particularly in mitosis. The T656 phosphorylation site, located within CLASP1’s conserved domain, is associated with Aurora B kinase activity, which peaks during metaphase-to-anaphase transitions. This modification is thought to regulate CLASP1’s interaction with microtubules and its role in stabilizing kinetochore fibers, ensuring proper chromosome alignment and segregation.
The Phospho-CLASP1(T656) antibody enables researchers to detect this specific post-translational modification, serving as a marker for studying mitotic regulation, spindle assembly checkpoint mechanisms, and errors in cell division linked to diseases like cancer. Validated in applications such as Western blotting and immunofluorescence, the antibody helps elucidate how CLASP1 phosphorylation impacts cellular responses to microtubule-targeting drugs or genomic instability. Its specificity for the phosphorylated form distinguishes it from pan-CLASP1 antibodies, making it valuable for dissecting signaling pathways involving Aurora kinases or other mitotic regulators. Studies using this antibody contribute to understanding cell cycle defects, potential therapeutic targets, and CLASP1’s broader roles in development and disease.
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