| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pumilio homolog 2, Pumilio-2, PUM2, KIAA0235, PUMH2 |
| Entrez GeneID | 23369 |
| WB Predicted band size | 114.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-162 amino acids from the N-terminal region of human PUM2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PUM2 (N-term)抗体的示例参考文献(均为虚构，仅供格式参考)：
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1. **文献名称**: "PUM2 N-terminal antibody validation in post-transcriptional regulation studies"
**作者**: Zhang et al.
**摘要**: 本研究验证了PUM2 (N-term)抗体的特异性，通过Western blot和免疫荧光证实其在多种细胞系中检测PUM2蛋白的表达，并应用于研究PUM2在mRNA稳定性调控中的作用。
2. **文献名称**: "PUM2 interacts with non-coding RNAs in neuronal differentiation"
**作者**: Martinez et al.
**摘要**: 利用PUM2 (N-term)抗体进行免疫共沉淀(Co-IP)，揭示了PUM2在神经分化过程中与特定lncRNAs的相互作用，调控神经元突触形成相关基因的表达。
3. **文献名称**: "Role of PUM2 in cancer metastasis and antibody-based detection"
**作者**: Kim et al.
**摘要**: 通过PUM2 (N-term)抗体的免疫组化分析，发现PUM2在乳腺癌组织中高表达，并与肿瘤转移相关通路(如EMT)的激活显著相关。
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*注：以上文献为模拟示例，实际文献请通过PubMed或Google Scholar检索关键词“PUM2 antibody N-term”获取。*
**Background of PUM2 (N-term) Antibody**
The PUM2 (Pumilio homolog 2) protein is a member of the evolutionarily conserved Pumilio/PUF family of RNA-binding proteins, which play critical roles in post-transcriptional gene regulation. PUM2 binds to specific sequences in target mRNAs, often within the 3' untranslated regions (UTRs), to regulate their stability, translation, or localization. It is involved in diverse biological processes, including neurodevelopment, stem cell maintenance, and stress response. Dysregulation of PUM2 has been linked to neurological disorders and cancers.
The PUM2 (N-term) antibody is a specific reagent designed to detect the N-terminal region of human PUM2. This region contains conserved domains essential for protein-protein interactions and functional regulation. The antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunohistochemistry to study PUM2 expression, localization, and interactions in cells and tissues. It is particularly valuable for distinguishing PUM2 from its paralog PUM1. given their structural similarities but distinct roles.
Validation of the antibody typically includes testing in knockout cell lines or tissues to confirm specificity. Researchers utilize this tool to explore PUM2's involvement in RNA metabolism, its pathological implications, and potential therapeutic targets. Its application extends to both basic research and disease-focused studies, aiding in elucidating PUM2's regulatory networks.
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