| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Insulin receptor, IR, CD220, Insulin receptor subunit alpha, Insulin receptor subunit beta, INSR |
| Entrez GeneID | 3643 |
| WB Predicted band size | 156.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This INSR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1185 of human INSR. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-INSR(Y1185)抗体的3篇参考文献及其摘要内容：
1. **文献名称**：*"Phosphorylation of Insulin Receptor Substrate-1 Tyr⁸⁹⁵: A Functional Study of Insulin Signaling in Vivo"*
**作者**：S. Boucher et al.
**摘要**：该研究利用Phospho-INSR(Y1185)抗体分析胰岛素受体酪氨酸磷酸化在胰岛素信号转导中的作用，发现Y1185位点的磷酸化对下游AKT/mTOR通路的激活至关重要。
2. **文献名称**：*"Development of Phosphospecific Antibodies to Insulin Receptor Tyrosine 1185 and Their Application in Metabolic Studies"*
**作者**：K. Tanaka et al.
**摘要**：作者报道了一种针对Y1185磷酸化位点的特异性抗体的开发，并通过免疫印迹和免疫组化验证其在糖尿病模型中对胰岛素受体活性检测的可靠性。
3. **文献名称**：*"Insulin Receptor Tyrosine Phosphorylation Sites Regulate Receptor Endocytosis and Signaling Specificity"*
**作者**：M. White et al.
**摘要**：研究使用Phospho-INSR(Y1185)抗体揭示了Y1185磷酸化在胰岛素受体内吞过程中的关键作用，并证明该位点异常与胰岛素抵抗相关。
(注：上述文献为示例，实际引用需核对真实数据库。Y1185编号可能因物种或受体亚型不同存在差异，建议通过PubMed或Google Scholar以关键词“Phospho-INSR(Y1185) antibody”检索最新文献。)
The Phospho-INSR(Y1185) antibody detects insulin receptor (INSR) when phosphorylated at tyrosine residue 1185. a key post-translational modification linked to receptor activation and downstream signaling. INSR is a transmembrane tyrosine kinase receptor composed of two α- and two β-subunits. Insulin binding induces autophosphorylation of specific tyrosine residues in the β-subunit’s kinase domain, including Y1185 (also numbered as Y1150 or Y1162 in some isoforms), which resides within the activation loop. This phosphorylation event triggers conformational changes that enhance the receptor’s kinase activity, enabling recruitment and phosphorylation of adaptor proteins like IRS-1/2. thereby initiating signaling cascades (e.g., PI3K/Akt and MAPK pathways) that regulate glucose uptake, metabolism, and cell growth.
Antibodies targeting Phospho-INSR(Y1185) are critical tools for studying insulin resistance, diabetes, and cancers with aberrant INSR signaling. They are commonly used in Western blotting, immunohistochemistry, or immunoprecipitation to assess receptor activation status in tissues or cell lines under varying insulin conditions or therapeutic interventions. Specificity validation via knockout/knockdown models or phosphatase treatment is essential to confirm signal fidelity. Research using this antibody has elucidated mechanisms of insulin-related pathologies and therapies targeting INSR activity.
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